biostudies-literatureLi Jthe Major Science and Technology Project Incubation Program of Bengbu Medical CollegeNational Natural Science Foundation of Chinathe Outstanding Youth Fund Project of the First Affiliated Hospital of Bengbu Medical Collegee13292https://www.ebi.ac.uk/biostudies/studies/S-EPMC9528773biostudies-literatureUnknown55(10)<h4>Objectives</h4>Crohn's disease (CD) mesenteric adipose tissue (MAT) inflammation affects enteritis through the interaction between the mesentery and intestine, and we previously found that poorly differentiated mesenteric adipocytes were related to its inflammatory features. Pygopus2 (Pygo2) is a key negative regulator of adipocyte differentiation. We aimed to determine whether Pygo2 participates in CD mesenteric lesions and whether Pygo2 knockdown would be beneficial in a CD model (Il-10^-/- mice).<h4>Methods</h4>Pygo2 expression in MAT from control and CD patients and Il-10^-/- mice was measured by immunohistochemistry. Lentiviral transfection was used to regulate Pygo2 expression in Il-10^-/- mice, and the effects on mesenteric adipocyte differentiationCell proliferationPygopus2 ameliorates mesenteric adipocyte poor differentiation to alleviate Crohn's disease -like colitis via the Axin2/GSK3β pathway.PMC95287732019byyfyyq022020byfy0032019byyfyjq018190207882070561Li JShi RLi QZuo LZhou YCheng YGe SYang ZHu JZhang XGeng ZWang YSong XNie WfalsePygopus2 ameliorates mesenteric adipocyte poor differentiation to alleviate Crohn's disease -like colitis via the Axin2/GSK3β pathway.<h4>Objectives</h4>Crohn's disease (CD) mesenteric adipose tissue (MAT) inflammation affects enteritis through the interaction between the mesentery and intestine, and we previously found that poorly differentiated mesenteric adipocytes were related to its inflammatory features. Pygopus2 (Pygo2) is a key negative regulator of adipocyte differentiation. We aimed to determine whether Pygo2 participates in CD mesenteric lesions and whether Pygo2 knockdown would be beneficial in a CD model (Il-10^-/- mice).<h4>Methods</h4>Pygo2 expression in MAT from control and CD patients and Il-10^-/- mice was measured by immunohistochemistry. Lentiviral transfection was used to regulate Pygo2 expression in Il-10^-/- mice, and the effects on mesenteric adipocyte differentiation2022-01-01T00:00:00Z2022 Oct2025-04-19T17:38:32.707Z2025-04-19T17:38:32.707ZS-EPMC95287733570787110.1111/cpr.13292