{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["61(5)"],"submitter":["Scicluna P"],"pubmed_abstract":["Abnormalities of the insulin‑like growth factor 2 <i>(IGF2)‑H19</i> locus with the overexpression of <i>IGF2</i> are frequent findings in adrenocortical carcinoma (ACC). The present study assessed the expression of RNAs and microRNAs (miRNAs/miRs) from the <i>IGF2‑H19</i> locus using PCR‑based methods in ACC and adrenocortical adenoma (ACA). The results were associated with proteomics data. <i>IGF2</i> was overexpressed in ACC, and its expression correlated with that of <i>miR‑483‑3p</i> and <i>miR‑483‑5p</i> hosted by <i>IGF2</i>. The downregulated expression of <i>H19</i> in ACC compared to ACA correlated with <i>miR‑675</i> expression hosted by <i>H19</i>. Several proteins exhibited an inverse correlation in expression and were predicted as targets of <i>miR‑483‑3p</i>, <i>miR‑483‑5p</i> or <i>miR‑675</i>. Subsets of these proteins were differentially expressed between ACC and ACA. These included several proteins involved in mitochondrial metabolism. Among the mitochondrial respiratory complexes, complex I and IV were significantly decreased in ACC compared to ACA. The protein expression of NADH:ubiquinone oxidoreductase subunit C1 (NDUFC1), a subunit of mitochondrial respiratory complex I, was further validated as being lower in ACC compared to ACA and normal adrenals. The silencing of <i>miR‑483‑5p</i> increased NDUFC1 protein expression and reduced both oxygen consumption and glycolysis rates. On the whole, the findings of the present study reveal the dysregulation of the <i>IGF2‑H19</i> locus and mitochondrial respiration in ACC. These findings may provide a basis for the further understanding of the pathogenesis of ACC and may have potential values for diagnostics and treatment."],"journal":["International journal of oncology"],"pagination":["140"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9529429"],"repository":["biostudies-literature"],"pubmed_title":["Altered expression of the <i>IGF2‑H19</i> locus and mitochondrial respiratory complexes in adrenocortical carcinoma."],"pmcid":["PMC9529429"],"pubmed_authors":["Caramuta S","Xu C","Gao J","Lui WO","Kjellman M","Scicluna P","Hoog A","Larsson C","Frobom R","Branstrom R","Akhtar M","Ekstrom TJ","Almgren M","Kjellin H","Shi H","Zedenius J"],"additional_accession":[]},"is_claimable":false,"name":"Altered expression of the <i>IGF2‑H19</i> locus and mitochondrial respiratory complexes in adrenocortical carcinoma.","description":"Abnormalities of the insulin‑like growth factor 2 <i>(IGF2)‑H19</i> locus with the overexpression of <i>IGF2</i> are frequent findings in adrenocortical carcinoma (ACC). The present study assessed the expression of RNAs and microRNAs (miRNAs/miRs) from the <i>IGF2‑H19</i> locus using PCR‑based methods in ACC and adrenocortical adenoma (ACA). The results were associated with proteomics data. <i>IGF2</i> was overexpressed in ACC, and its expression correlated with that of <i>miR‑483‑3p</i> and <i>miR‑483‑5p</i> hosted by <i>IGF2</i>. The downregulated expression of <i>H19</i> in ACC compared to ACA correlated with <i>miR‑675</i> expression hosted by <i>H19</i>. Several proteins exhibited an inverse correlation in expression and were predicted as targets of <i>miR‑483‑3p</i>, <i>miR‑483‑5p</i> or <i>miR‑675</i>. Subsets of these proteins were differentially expressed between ACC and ACA. These included several proteins involved in mitochondrial metabolism. Among the mitochondrial respiratory complexes, complex I and IV were significantly decreased in ACC compared to ACA. The protein expression of NADH:ubiquinone oxidoreductase subunit C1 (NDUFC1), a subunit of mitochondrial respiratory complex I, was further validated as being lower in ACC compared to ACA and normal adrenals. The silencing of <i>miR‑483‑5p</i> increased NDUFC1 protein expression and reduced both oxygen consumption and glycolysis rates. On the whole, the findings of the present study reveal the dysregulation of the <i>IGF2‑H19</i> locus and mitochondrial respiration in ACC. These findings may provide a basis for the further understanding of the pathogenesis of ACC and may have potential values for diagnostics and treatment.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Nov","modification":"2025-04-26T15:36:30.297Z","creation":"2025-04-06T14:57:07.233Z"},"accession":"S-EPMC9529429","cross_references":{"pubmed":["36169175"],"doi":["10.3892/ijo.2022.5430"]}}