biostudies-literatureXing LGovernment of Jiangsu ProvincePriority Academic Program Development of Jiangsu Higher Education InstitutionsNational Natural Science Foundation of ChinaNational Key Research and Development Program of China102452https://www.ebi.ac.uk/biostudies/studies/S-EPMC9530849biostudies-literatureUnknown298(10)The pMN domain is a restricted domain in the ventral spinal cord, defined by the expression of the olig2 gene. Though it is known that the pMN progenitor cells can sequentially generate motor neurons and oligodendrocytes, the lineages of these progenitors are controversial and how their progeny are generated is not well understood. Using single-cell RNA sequencing, here, we identified a previously unknown heterogeneity among pMN progenitors with distinct fates and molecular signatures in zebrafish. Notably, we characterized two distinct motor neuron lineages using bioinformatic analysis. We then went on to investigate specific molecular programs that regulate neural progenitor fate transition. We validated experimentally that expression of the transcription factor myt1 (myelin transcriptioThe Journal of biological chemistryExpression of myelin transcription factor 1 and lamin B receptor mediate neural progenitor fate transition in the zebrafish spinal cord pMN domain.PMC95308492017YFA010470431872773BE20206673207099881701127Li HXing LChai RChen GLai BLin JWang YWang JSun JfalseExpression of myelin transcription factor 1 and lamin B receptor mediate neural progenitor fate transition in the zebrafish spinal cord pMN domain.The pMN domain is a restricted domain in the ventral spinal cord, defined by the expression of the olig2 gene. Though it is known that the pMN progenitor cells can sequentially generate motor neurons and oligodendrocytes, the lineages of these progenitors are controversial and how their progeny are generated is not well understood. Using single-cell RNA sequencing, here, we identified a previously unknown heterogeneity among pMN progenitors with distinct fates and molecular signatures in zebrafish. Notably, we characterized two distinct motor neuron lineages using bioinformatic analysis. We then went on to investigate specific molecular programs that regulate neural progenitor fate transition. We validated experimentally that expression of the transcription factor myt1 (myelin transcriptio2022-01-01T00:00:00Z2022 Oct2026-07-14T17:36:44.766Z2026-06-22T03:08:32.748ZS-EPMC95308493606399810.1016/j.jbc.2022.102452