biostudies-literatureYoshioka YCore Research for Evolutional Science and TechnologyJapan Agency for Medical Research and DevelopmentCenter of Innovation ProgramJapan Society for the Promotion of Science3498-3509https://www.ebi.ac.uk/biostudies/studies/S-EPMC9530877biostudies-literatureUnknown113(10)Early detection of pancreatic ductal adenocarcinoma (PDAC) is essential for improving patient survival rates, and noninvasive biomarkers are urgently required to identify patients who are eligible for curative surgery. Here, we examined extracellular vesicles (EVs) from the serum of PDAC patients to determine their ability to detect early-stage disease. EV-associated proteins purified by ultracentrifugation and affinity columns underwent proteomic analysis to identify novel PDAC markers G protein-coupled receptor class C group 5 member C (GPRC5C) and epidermal growth factor receptor pathway substrate 8 (EPS8). To verify the potency of GPRC5C- or EPS8-positive EVs as PDAC biomarkers, we analyzed EVs from PDAC patient blood samples using ultracentrifugation in two different cohorts (a total Cancer scienceCirculating cancer-associated extracellular vesicles as early detection and recurrence biomarkers for pancreatic cancer.PMC9530877JP20cm0106402JPMJCR19H115H05791Mori MItoi TEguchi HNakatsura TDoki YKuroda MSaito KIshii HOchiya TShimomura MYoshioka YfalseCirculating cancer-associated extracellular vesicles as early detection and recurrence biomarkers for pancreatic cancer.Early detection of pancreatic ductal adenocarcinoma (PDAC) is essential for improving patient survival rates, and noninvasive biomarkers are urgently required to identify patients who are eligible for curative surgery. Here, we examined extracellular vesicles (EVs) from the serum of PDAC patients to determine their ability to detect early-stage disease. EV-associated proteins purified by ultracentrifugation and affinity columns underwent proteomic analysis to identify novel PDAC markers G protein-coupled receptor class C group 5 member C (GPRC5C) and epidermal growth factor receptor pathway substrate 8 (EPS8). To verify the potency of GPRC5C- or EPS8-positive EVs as PDAC biomarkers, we analyzed EVs from PDAC patient blood samples using ultracentrifugation in two different cohorts (a total 2022-01-01T00:00:00Z2022 Oct2025-04-04T23:20:14.54Z2025-04-04T23:20:14.54ZS-EPMC95308773584889610.1111/cas.15500