biostudies-literatureMa YNational Natural Science Foundation of China30https://www.ebi.ac.uk/biostudies/studies/S-EPMC9531454biostudies-literatureUnknown55(1)<h4>Background</h4>Xenotransplantation has been primarily performed using fresh donor tissue to study testicular development for about 20 years, and whether the cultured tissue would be a suitable donor is unclear. In this study, we combined testicular culture and xenotransplantation into an integrative model and explored whether immature testicular tissue would survive and continue to develop in this model.<h4>Methods</h4>In the new integrative model group, the testes of neonatal rats on postnatal day 8 (PND 8) were cultured for 4 days ex vivo and then were transplanted under the dorsal skin of castrated nude mice. The xenografted testes were resected on the 57th day after xenotransplantation and the testes of rats in the control group were harvested on PND 69. The survival state of testiBiological researchImmature rat testis sustained long-term development using an integrative model.PMC953145481801436Li HChen JMa YXu FZhang LWang ZChong TfalseImmature rat testis sustained long-term development using an integrative model.<h4>Background</h4>Xenotransplantation has been primarily performed using fresh donor tissue to study testicular development for about 20 years, and whether the cultured tissue would be a suitable donor is unclear. In this study, we combined testicular culture and xenotransplantation into an integrative model and explored whether immature testicular tissue would survive and continue to develop in this model.<h4>Methods</h4>In the new integrative model group, the testes of neonatal rats on postnatal day 8 (PND 8) were cultured for 4 days ex vivo and then were transplanted under the dorsal skin of castrated nude mice. The xenografted testes were resected on the 57th day after xenotransplantation and the testes of rats in the control group were harvested on PND 69. The survival state of testi2022-01-01T00:00:00Z2022 Oct2025-04-22T21:54:05.619Z2025-02-19T01:28:15.799ZS-EPMC95314543619594710.1186/s40659-022-00398-y