{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Doerfler R"],"funding":["NIBIB NIH HHS","NICHD NIH HHS","National Institutes of Health"],"pagination":["e2200090"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9532377"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["66(19)"],"pubmed_abstract":["<h4>Scope</h4>Human breast milk contains a variety of cell types that have potential roles in infant immunity and development. One challenge associates with defining the purpose(s) of milk cells in the infant is a poor understanding of the effect of digestion on cell fate.<h4>Methods and results</h4>This study first demonstrates that milk cell death occurs after gastric digestion in mice. Then flow cytometry and RT-PCR are used to understand the mechanism of human milk cell death and quantify live cell types before and after simulated gastric digestion. This study finds that digestion in simulated gastric fluid for 30 min reduces cell viability from 72% to 27%, with most cell death is caused by the acidic pH. The primary mechanism of cell death is caspase-mediated apoptosis. The non-cellul"],"journal":["Molecular nutrition & food research"],"pubmed_title":["The Effect of Infant Gastric Digestion on Human Maternal Milk Cells."],"pmcid":["PMC9532377"],"funding_grant_id":["DP2‐HD098860","DP2 HD098860","F32 EB009623","F32‐EB029345‐01","F32 EB029345"],"pubmed_authors":["Doerfler R","Melamed JR","Whitehead KA"],"additional_accession":[]},"is_claimable":false,"name":"The Effect of Infant Gastric Digestion on Human Maternal Milk Cells.","description":"<h4>Scope</h4>Human breast milk contains a variety of cell types that have potential roles in infant immunity and development. One challenge associates with defining the purpose(s) of milk cells in the infant is a poor understanding of the effect of digestion on cell fate.<h4>Methods and results</h4>This study first demonstrates that milk cell death occurs after gastric digestion in mice. Then flow cytometry and RT-PCR are used to understand the mechanism of human milk cell death and quantify live cell types before and after simulated gastric digestion. This study finds that digestion in simulated gastric fluid for 30 min reduces cell viability from 72% to 27%, with most cell death is caused by the acidic pH. The primary mechanism of cell death is caspase-mediated apoptosis. The non-cellul","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2026-07-14T20:10:24.651Z","creation":"2025-04-19T22:29:39.276Z"},"accession":"S-EPMC9532377","cross_references":{"pubmed":["35984112"],"doi":["10.1002/mnfr.202200090"]}}