{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pitter KL"],"funding":["NCI NIH HHS","NIH HHS"],"pagination":["3549-3560"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9532381"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["82(19)"],"pubmed_abstract":["Intratumoral heterogeneity and cellular plasticity have emerged as hallmarks of cancer, including pancreatic ductal adenocarcinoma (PDAC). As PDAC portends a dire prognosis, a better understanding of the mechanisms underpinning cellular diversity in PDAC is crucial. Here, we investigated the cellular heterogeneity of PDAC cancer cells across a range of in vitro and in vivo growth conditions using single-cell genomics. Heterogeneity contracted significantly in two-dimensional and three-dimensional cell culture models but was restored upon orthotopic transplantation. Orthotopic transplants reproducibly acquired cell states identified in autochthonous PDAC tumors, including a basal state exhibiting coexpression and coaccessibility of epithelial and mesenchymal genes. Lineage tracing combined "],"journal":["Cancer research"],"pubmed_title":["Systematic Comparison of Pancreatic Ductal Adenocarcinoma Models Identifies a Conserved Highly Plastic Basal Cell State."],"pmcid":["PMC9532381"],"funding_grant_id":["T32 CA009207","P30 CA008748","R37 CA244911","DP5 OD031864"],"pubmed_authors":["Wu K","Pe'er D","Bhutkar A","Ferrena A","Singhal A","Hudson A","Blum M","de Stanchina E","Tammela T","Chaligne R","Grbovic-Huezo O","Joost S","Lecomte N","Iacobuzio-Donahue CA","Pitter KL","Holm M"],"additional_accession":[]},"is_claimable":false,"name":"Systematic Comparison of Pancreatic Ductal Adenocarcinoma Models Identifies a Conserved Highly Plastic Basal Cell State.","description":"Intratumoral heterogeneity and cellular plasticity have emerged as hallmarks of cancer, including pancreatic ductal adenocarcinoma (PDAC). As PDAC portends a dire prognosis, a better understanding of the mechanisms underpinning cellular diversity in PDAC is crucial. Here, we investigated the cellular heterogeneity of PDAC cancer cells across a range of in vitro and in vivo growth conditions using single-cell genomics. Heterogeneity contracted significantly in two-dimensional and three-dimensional cell culture models but was restored upon orthotopic transplantation. Orthotopic transplants reproducibly acquired cell states identified in autochthonous PDAC tumors, including a basal state exhibiting coexpression and coaccessibility of epithelial and mesenchymal genes. Lineage tracing combined ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2026-05-28T03:54:22.126Z","creation":"2024-11-21T05:41:55.975Z"},"accession":"S-EPMC9532381","cross_references":{"pubmed":["35952360"],"doi":["10.1158/0008-5472.CAN-22-1742","10.1158/0008-5472.can-22-1742"]}}