{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sanchez Sanchez G"],"funding":["Fondation Jaumotte-Dumoulin ARC (ULB) grant Win2Wal grant from the Walloon region","EC | European Regional Development Fund (Europski Fond za Regionalni Razvoj)","Fonds De La Recherche Scientifique - FNRS (Belgian National Fund for Scientific Research)","Fonds De La Recherche Scientifique - FNRS","EC | European Regional Development Fund"],"pagination":["5842"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9532436"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["Developmental thymic waves of innate-like and adaptive-like γδ T cells have been described, but the current understanding of γδ T cell development is mainly limited to mouse models. Here, we combine single cell (sc) RNA gene expression and sc γδ T cell receptor (TCR) sequencing on fetal and pediatric γδ thymocytes in order to understand the ontogeny of human γδ T cells. Mature fetal γδ thymocytes (both the Vγ9Vδ2 and nonVγ9Vδ2 subsets) are committed to either a type 1, a type 3 or a type 2-like effector fate displaying a wave-like pattern depending on gestation age, and are enriched for public CDR3 features upon maturation. Strikingly, these effector modules express different CDR3 sequences and follow distinct developmental trajectories. In contrast, the pediatric thymus generates only a s"],"journal":["Nature communications"],"pubmed_title":["Identification of distinct functional thymic programming of fetal and pediatric human γδ thymocytes via single-cell analysis."],"pmcid":["PMC9532436"],"funding_grant_id":["411132-957270","J.0225.20"],"pubmed_authors":["Papadopoulou M","Porco S","Fan Y","Azouz A","Vandekerckhove B","Libert F","Chan JKY","Sanchez Sanchez G","Ginhoux F","Goriely S","Mishra A","Vermijlen D","Tafesse Y","Verdebout I"],"additional_accession":[]},"is_claimable":false,"name":"Identification of distinct functional thymic programming of fetal and pediatric human γδ thymocytes via single-cell analysis.","description":"Developmental thymic waves of innate-like and adaptive-like γδ T cells have been described, but the current understanding of γδ T cell development is mainly limited to mouse models. Here, we combine single cell (sc) RNA gene expression and sc γδ T cell receptor (TCR) sequencing on fetal and pediatric γδ thymocytes in order to understand the ontogeny of human γδ T cells. Mature fetal γδ thymocytes (both the Vγ9Vδ2 and nonVγ9Vδ2 subsets) are committed to either a type 1, a type 3 or a type 2-like effector fate displaying a wave-like pattern depending on gestation age, and are enriched for public CDR3 features upon maturation. Strikingly, these effector modules express different CDR3 sequences and follow distinct developmental trajectories. In contrast, the pediatric thymus generates only a s","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-04T09:25:07.268Z","creation":"2025-04-04T09:25:07.268Z"},"accession":"S-EPMC9532436","cross_references":{"pubmed":["36195611"],"doi":["10.1038/s41467-022-33488-2"]}}