biostudies-literatureUnknown13Clua JHeterotrimeric Nuclear Factor Y (NF-Y) transcription factors are key regulators of the symbiotic program that controls rhizobial infection and nodule organogenesis. Using a yeast two-hybrid screening, we identified a putative protein kinase of <i>Phaseolus vulgaris</i> that interacts with the C subunit of the NF-Y complex. Physical interaction between NF-YC1 Interacting Protein Kinase (NIPK) and NF-YC1 occurs in the cytoplasm and the plasma membrane. Only one of the three canonical amino acids predicted to be required for catalytic activity is conserved in NIPK and its putative homologs from lycophytes to angiosperms, indicating that NIPK is an evolutionary conserved pseudokinase. Post-transcriptional silencing on <i>NIPK</i> affected infection and nodule organogenesis, suggesting NIPK is Frontiers in plant science992543https://www.ebi.ac.uk/biostudies/studies/S-EPMC9532615biostudies-literatureNIPK, a protein pseudokinase that interacts with the C subunit of the transcription factor NF-Y, is involved in rhizobial infection and nodule organogenesis.PMC9532615Clua JBlanco FARoda CZanetti MEBattaglia MERipodas CfalseNIPK, a protein pseudokinase that interacts with the C subunit of the transcription factor NF-Y, is involved in rhizobial infection and nodule organogenesis.Heterotrimeric Nuclear Factor Y (NF-Y) transcription factors are key regulators of the symbiotic program that controls rhizobial infection and nodule organogenesis. Using a yeast two-hybrid screening, we identified a putative protein kinase of <i>Phaseolus vulgaris</i> that interacts with the C subunit of the NF-Y complex. Physical interaction between NF-YC1 Interacting Protein Kinase (NIPK) and NF-YC1 occurs in the cytoplasm and the plasma membrane. Only one of the three canonical amino acids predicted to be required for catalytic activity is conserved in NIPK and its putative homologs from lycophytes to angiosperms, indicating that NIPK is an evolutionary conserved pseudokinase. Post-transcriptional silencing on <i>NIPK</i> affected infection and nodule organogenesis, suggesting NIPK is 2022-01-01T00:00:00Z20222025-05-18T13:02:54.995Z2024-10-18T11:23:43.189ZS-EPMC95326153621234010.3389/fpls.2022.992543