biostudies-literatureUnknown24(5)Nakazawa SThe linear ubiquitin chain assembly complex (LUBAC), which is composed of RING finger protein 31 (RNF31), RANBP2-type and C3HC4-type zinc finger containing 1 and SHANK-associated RH domain interactor subunits, is the only ubiquitin ligase to generate Met1-linked linear ubiquitin chains. Linear ubiquitin chains regulate canonical NF-κB activation and cell death. Single nucleotide polymorphisms in RNF31, such as Q584H and Q622L, are known to cause the activated B cell-like subtype of diffuse large B cell lymphoma (ABC-DLBCL) because of enhanced LUBAC-mediated NF-κB activation. The present study identified a novel Q622H polymorphism of RNF31 in two patients with lung cancer, one of whom had concurrent ABC-DLBCL. Immunohistochemical analyses revealed that although the expression of RNF31 was eOncology letters394https://www.ebi.ac.uk/biostudies/studies/S-EPMC9533369biostudies-literatureIdentification and molecular analysis of RNF31 Q622H germline polymorphism.PMC9533369Tokunaga FShirabe KNakazawa SKaira KNobusawa SKawabata-Iwakawa RMamiya RSato YOikawa DYajima TSasaki AfalseIdentification and molecular analysis of RNF31 Q622H germline polymorphism.The linear ubiquitin chain assembly complex (LUBAC), which is composed of RING finger protein 31 (RNF31), RANBP2-type and C3HC4-type zinc finger containing 1 and SHANK-associated RH domain interactor subunits, is the only ubiquitin ligase to generate Met1-linked linear ubiquitin chains. Linear ubiquitin chains regulate canonical NF-κB activation and cell death. Single nucleotide polymorphisms in RNF31, such as Q584H and Q622L, are known to cause the activated B cell-like subtype of diffuse large B cell lymphoma (ABC-DLBCL) because of enhanced LUBAC-mediated NF-κB activation. The present study identified a novel Q622H polymorphism of RNF31 in two patients with lung cancer, one of whom had concurrent ABC-DLBCL. Immunohistochemical analyses revealed that although the expression of RNF31 was e2022-01-01T00:00:00Z2022 Nov2026-05-27T11:43:34.233Z2024-11-08T14:35:27.18ZS-EPMC95333693627648110.3892/ol.2022.13514