{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Cai Y"],"funding":["Natural Science Foundation of Fujian Province","National Natural Science Foundation of China"],"pagination":["5868"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9534904"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["13(1)"],"pubmed_abstract":["DNA damage plays a critical role in biology and diseases; however, how different types of DNA lesions affect cellular functions is far from clear mostly due to the paucity of high-resolution methods that can map their locations in complex genomes, such as those of mammals. Here, we present the development and validation of SSiNGLe-AP method, which can map a common type of DNA damage, abasic (AP) sites, in a genome-wide and high-resolution manner. We apply this method to six different tissues of mice with different ages and human cancer cell lines. We find a nonrandom distribution of AP sites in the mammalian genome that exhibits dynamic enrichment at specific genomic locations, including single-nucleotide hotspots, and is significantly influenced by gene expression, age and tissue type in "],"journal":["Nature communications"],"pubmed_title":["Complex genomic patterns of abasic sites in mammalian DNA revealed by a high-resolution SSiNGLe-AP method."],"pmcid":["PMC9534904"],"funding_grant_id":["32150710525","2020J02006"],"pubmed_authors":["Cao H","Zhang Y","Kapranov P","Cai Y","Wang F"],"additional_accession":[]},"is_claimable":false,"name":"Complex genomic patterns of abasic sites in mammalian DNA revealed by a high-resolution SSiNGLe-AP method.","description":"DNA damage plays a critical role in biology and diseases; however, how different types of DNA lesions affect cellular functions is far from clear mostly due to the paucity of high-resolution methods that can map their locations in complex genomes, such as those of mammals. Here, we present the development and validation of SSiNGLe-AP method, which can map a common type of DNA damage, abasic (AP) sites, in a genome-wide and high-resolution manner. We apply this method to six different tissues of mice with different ages and human cancer cell lines. We find a nonrandom distribution of AP sites in the mammalian genome that exhibits dynamic enrichment at specific genomic locations, including single-nucleotide hotspots, and is significantly influenced by gene expression, age and tissue type in ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2026-07-14T17:36:45.729Z","creation":"2025-04-19T17:36:06.432Z"},"accession":"S-EPMC9534904","cross_references":{"pubmed":["36198706"],"doi":["10.1038/s41467-022-33594-1"]}}