biostudies-literaturePark YNational Office for Research, Development and InnovationEuropean Research Councile2203444https://www.ebi.ac.uk/biostudies/studies/S-EPMC9534983biostudies-literatureUnknown9(28)Metal sulfides are a common group of extracellular bacterial biominerals. However, only a few cases of intracellular biomineralization are reported in this group, mostly limited to greigite (Fe₃ S₄ ) in magnetotactic bacteria. Here, a previously unknown periplasmic biomineralization of copper sulfide produced by the magnetotactic bacterium Desulfamplus magnetovallimortis strain BW-1, a species known to mineralize greigite (Fe₃ S₄ ) and magnetite (Fe₃ O₄ ) in the cytoplasm is reported. BW-1 produces hundreds of spherical nanoparticles, composed of 1-2 nm substructures of a poorly crystalline hexagonal copper sulfide structure that remains in a thermodynamically unstable state. The particles appear to be surrounded by an organAdvanced science (Weinheim, Baden-Wurttemberg, Germany)Periplasmic Bacterial Biomineralization of Copper Sulfide Nanoparticles.PMC9534983NKFIH-471-3/2021848339Monteil CLFaivre DPark YArmengaud JGal ALefevre CTEyal ZChevrier DMPosfai MArnoux PPekker PfalsePeriplasmic Bacterial Biomineralization of Copper Sulfide Nanoparticles.Metal sulfides are a common group of extracellular bacterial biominerals. However, only a few cases of intracellular biomineralization are reported in this group, mostly limited to greigite (Fe₃ S₄ ) in magnetotactic bacteria. Here, a previously unknown periplasmic biomineralization of copper sulfide produced by the magnetotactic bacterium Desulfamplus magnetovallimortis strain BW-1, a species known to mineralize greigite (Fe₃ S₄ ) and magnetite (Fe₃ O₄ ) in the cytoplasm is reported. BW-1 produces hundreds of spherical nanoparticles, composed of 1-2 nm substructures of a poorly crystalline hexagonal copper sulfide structure that remains in a thermodynamically unstable state. The particles appear to be surrounded by an organ2022-01-01T00:00:00Z2022 Oct2025-04-05T11:38:11.404Z2025-04-05T11:38:11.404ZS-EPMC95349833597541910.1002/advs.202203444