{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["4(10)"],"submitter":["Loo Y"],"pubmed_abstract":["Organotypic skin cultures represent in vitro models of skin which can be used for disease modeling, tissue engineering, and screening applications. Non-human collagen is currently the gold standard material used for the construction of the supporting matrix, however, its clinical applications are limited due to its xenogeneic origin. We have developed a novel peptide hydrogel-based skin construct that shows a pluristratified epidermis, basement membrane, and dermal compartment after 3 weeks of in vitro culture. Peptide-based constructs were compared to collagen-based constructs and stratification marker expression was histologically higher in peptide constructs than in collagen constructs. Transepithelial electrical resistance also showed mature barrier function in peptide constructs. This study presents a novel application of the self-assembling peptide hydrogel in a defined xeno-free in vitro system."],"journal":["FASEB bioAdvances"],"pagination":["631-637"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9536085"],"repository":["biostudies-literature"],"pubmed_title":["Xeno-free self-assembling peptide scaffolds for building 3D organotypic skin cultures."],"pmcid":["PMC9536085"],"pubmed_authors":["Hauser CAE","Benny P","Wan ACA","Loo Y","Lane EB"],"additional_accession":[]},"is_claimable":false,"name":"Xeno-free self-assembling peptide scaffolds for building 3D organotypic skin cultures.","description":"Organotypic skin cultures represent in vitro models of skin which can be used for disease modeling, tissue engineering, and screening applications. Non-human collagen is currently the gold standard material used for the construction of the supporting matrix, however, its clinical applications are limited due to its xenogeneic origin. We have developed a novel peptide hydrogel-based skin construct that shows a pluristratified epidermis, basement membrane, and dermal compartment after 3 weeks of in vitro culture. Peptide-based constructs were compared to collagen-based constructs and stratification marker expression was histologically higher in peptide constructs than in collagen constructs. Transepithelial electrical resistance also showed mature barrier function in peptide constructs. This study presents a novel application of the self-assembling peptide hydrogel in a defined xeno-free in vitro system.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-05T20:18:07.015Z","creation":"2025-04-05T20:18:07.015Z"},"accession":"S-EPMC9536085","cross_references":{"pubmed":["36238363"],"doi":["10.1096/fba.2022-00026"]}}