biostudies-literatureUnknown86(4)Tran HThe goal of this study was to determine if seasonality of rotavirus A, B, and C infection is present in Ontario and Quebec swine herds by investigating submissions to a diagnostic laboratory. Samples (N = 1557) within 755 case submissions from Canadian swine herds between 2016 and 2020 were tested for rotaviruses A, B, and C using a real-time polymerase-chain reaction assay and described. Data from Ontario and Quebec were additionally analyzed using boxplots, 6-week rolling averages, time-series decomposition, and negative binomial regression models. Percentage positivity of submissions for rotaviruses A, B, and C were discovered to be highest in nursery/weaner (<i>n</i> = 100, 94.0%, 60.0%, 80.0%) and grower/finisher (<i>n</i> = 13, 84.6%, 46.2%, 61.5%) pigs and lowest in gilt/sow (<i>n</Canadian journal of veterinary research = Revue canadienne de recherche veterinaire241-253https://www.ebi.ac.uk/biostudies/studies/S-EPMC9536357biostudies-literatureAssessment of seasonality of rotavirus PCR detection in swine from Ontario and Quebec between 2016-2020 using submissions to a diagnostic laboratory.PMC9536357Poljak ZTran HOjkic DFriendship RfalseAssessment of seasonality of rotavirus PCR detection in swine from Ontario and Quebec between 2016-2020 using submissions to a diagnostic laboratory.The goal of this study was to determine if seasonality of rotavirus A, B, and C infection is present in Ontario and Quebec swine herds by investigating submissions to a diagnostic laboratory. Samples (N = 1557) within 755 case submissions from Canadian swine herds between 2016 and 2020 were tested for rotaviruses A, B, and C using a real-time polymerase-chain reaction assay and described. Data from Ontario and Quebec were additionally analyzed using boxplots, 6-week rolling averages, time-series decomposition, and negative binomial regression models. Percentage positivity of submissions for rotaviruses A, B, and C were discovered to be highest in nursery/weaner (<i>n</i> = 100, 94.0%, 60.0%, 80.0%) and grower/finisher (<i>n</i> = 13, 84.6%, 46.2%, 61.5%) pigs and lowest in gilt/sow (<i>n</2022-01-01T00:00:00Z2022 Oct2025-08-15T03:07:01.331Z2025-04-06T16:10:44.492ZS-EPMC953635736211211