{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Pelegri-Martinez E"],"funding":["University of the Basque Country","Eusko Jaurlaritza"],"pagination":["3534-3545"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9537992"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["133(6)"],"pubmed_abstract":["<h4>Introduction</h4>Quantitative reverse transcription PCR (RT-qPCR) is the leading tool to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Given that it will almost certainly continue to coexist with other respiratory viruses in the coming years, our study aimed to design a multiplex PCR system not affected by supplier outages and with reduced cost compared to the existing commercially available kits.<h4>Methods and results</h4>In this study, combinations of four primers/probe sets were used to construct a flexible RT-qPCR assay which is capable of discriminating between SARS-CoV-2 and the seasonal human coronavirus HCoV-OC43, or even influenza A virus. Additionally, the human RPP30 gene was used as an internal control. To demonstrate the robustness of the assay, it was applied to a collection of 150 clinical samples. The results showed 100% sensitivity and specificity compared to the automatized system used at the hospital and were better when indeterminate samples were analysed.<h4>Conclusions</h4>This study provides an efficient method for the simultaneous detection of SARS-CoV-2, HCoV-OC43 and influenza A virus, and its efficacy has been tested on clinical samples showing outstanding results.<h4>Significance and impact of the study</h4>The multiplex RT-qPCR design offers an accessible and economical alternative to commercial detection kits for hospitals and laboratories with limited economic resources or facing situations of supply shortage."],"journal":["Journal of applied microbiology"],"pubmed_title":["Flexible multiplex PCR to detect SARS-CoV-2, coronavirus OC43 and influenza A virus in nasopharyngeal swab samples."],"pmcid":["PMC9537992"],"funding_grant_id":["IT1362-19","2020333042"],"pubmed_authors":["Aranzamendi M","Pelegri-Martinez E","Dominguez-Monedero A","Ramirez-Garcia A","Martin-Souto L","Martinez O","Gallego M","Arana-Arri E","Guruceaga X","Rementeria A","Abad-Diaz-de-Cerio A"],"additional_accession":[]},"is_claimable":false,"name":"Flexible multiplex PCR to detect SARS-CoV-2, coronavirus OC43 and influenza A virus in nasopharyngeal swab samples.","description":"<h4>Introduction</h4>Quantitative reverse transcription PCR (RT-qPCR) is the leading tool to detect severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Given that it will almost certainly continue to coexist with other respiratory viruses in the coming years, our study aimed to design a multiplex PCR system not affected by supplier outages and with reduced cost compared to the existing commercially available kits.<h4>Methods and results</h4>In this study, combinations of four primers/probe sets were used to construct a flexible RT-qPCR assay which is capable of discriminating between SARS-CoV-2 and the seasonal human coronavirus HCoV-OC43, or even influenza A virus. Additionally, the human RPP30 gene was used as an internal control. To demonstrate the robustness of the assay, it was applied to a collection of 150 clinical samples. The results showed 100% sensitivity and specificity compared to the automatized system used at the hospital and were better when indeterminate samples were analysed.<h4>Conclusions</h4>This study provides an efficient method for the simultaneous detection of SARS-CoV-2, HCoV-OC43 and influenza A virus, and its efficacy has been tested on clinical samples showing outstanding results.<h4>Significance and impact of the study</h4>The multiplex RT-qPCR design offers an accessible and economical alternative to commercial detection kits for hospitals and laboratories with limited economic resources or facing situations of supply shortage.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-04T22:32:41.187Z","creation":"2025-02-19T03:23:02.221Z"},"accession":"S-EPMC9537992","cross_references":{"pubmed":["35988051"],"doi":["10.1111/jam.15788"]}}