biostudies-literatureUnknown12Wang YThe microsporidian <i>Enterocytozoon hepatopenaei</i> (EHP) has become a critical threat to the global shrimp aquaculture industry, thus necessitating early detection by screening. Development of a rapid and accurate assay is crucial both for the active surveillance and for the assessment of shrimp with EHP infection. In the present study, a distinct strain of <i>E</i>. <i>hepatopenaei</i> (EHP <i>_Mr</i> ) was found in <i>Macrobrachium rosenbergii</i>. The SWP1 gene analysis revealed it was a new genotype that differed with the common strain isolated from the <i>Litopenaeus vannamei</i> (EHP <i>_Lv</i> ). A nested SWP-PCR method was modified to fix the bug that the original inner primers could not recognize the EHP <i>_Mr</i> strain. The redesigned inner primFrontiers in cellular and infection microbiology1013016https://www.ebi.ac.uk/biostudies/studies/S-EPMC9538563biostudies-literatureA modification of nested PCR method for detection of <i>Enterocytozoon hepatopenaei</i> (EHP) in giant freshwater prawn <i>Macrobrachium rosenbergii</i>.PMC9538563Li XTan HFang WXiang YWang YYin MYing NZhou JLiu WYe JfalseA modification of nested PCR method for detection of <i>Enterocytozoon hepatopenaei</i> (EHP) in giant freshwater prawn <i>Macrobrachium rosenbergii</i>.The microsporidian <i>Enterocytozoon hepatopenaei</i> (EHP) has become a critical threat to the global shrimp aquaculture industry, thus necessitating early detection by screening. Development of a rapid and accurate assay is crucial both for the active surveillance and for the assessment of shrimp with EHP infection. In the present study, a distinct strain of <i>E</i>. <i>hepatopenaei</i> (EHP <i>_Mr</i> ) was found in <i>Macrobrachium rosenbergii</i>. The SWP1 gene analysis revealed it was a new genotype that differed with the common strain isolated from the <i>Litopenaeus vannamei</i> (EHP <i>_Lv</i> ). A nested SWP-PCR method was modified to fix the bug that the original inner primers could not recognize the EHP <i>_Mr</i> strain. The redesigned inner prim2022-01-01T00:00:00Z20222025-05-29T16:10:27.042Z2025-04-05T12:17:25.541ZS-EPMC95385633621197210.3389/fcimb.2022.1013016