{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sana S"],"funding":["Department of Biotechnology (DBT)-Indian Institute of Science Partnership Program","Wellcome Trust India Alliance Fellowship"],"pagination":["e202203127"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9539458"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["221(12)"],"pubmed_abstract":["In animal cells, spindle elongation during anaphase is temporally coupled with cleavage furrow formation. Spindle elongation during anaphase is regulated by NuMA/dynein/dynactin complexes that occupy the polar region of the cell membrane and are excluded from the equatorial membrane. How NuMA/dynein/dynactin are excluded from the equatorial membrane and the biological significance of this exclusion remains unknown. Here, we show that the centralspindlin (Cyk4/Mklp1) and its interacting partner RhoGEF Ect2 are required for NuMA/dynein/dynactin exclusion from the equatorial cell membrane. The Ect2-based (Ect2/Cyk4/Mklp1) and NuMA-based (NuMA/dynein/dynactin) complexes occupy mutually exclusive membrane surfaces during anaphase. The equatorial membrane enrichment of Ect2-based complexes is es"],"journal":["The Journal of cell biology"],"pubmed_title":["Membrane compartmentalization of Ect2/Cyk4/Mklp1 and NuMA/dynein regulates cleavage furrow formation."],"pmcid":["PMC9539458"],"funding_grant_id":["BT/PR36084/BRB/10/1857/2020","IA/I/15/2/502077"],"pubmed_authors":["Kotak S","Rajeevan A","Sana S"],"additional_accession":[]},"is_claimable":false,"name":"Membrane compartmentalization of Ect2/Cyk4/Mklp1 and NuMA/dynein regulates cleavage furrow formation.","description":"In animal cells, spindle elongation during anaphase is temporally coupled with cleavage furrow formation. Spindle elongation during anaphase is regulated by NuMA/dynein/dynactin complexes that occupy the polar region of the cell membrane and are excluded from the equatorial membrane. How NuMA/dynein/dynactin are excluded from the equatorial membrane and the biological significance of this exclusion remains unknown. Here, we show that the centralspindlin (Cyk4/Mklp1) and its interacting partner RhoGEF Ect2 are required for NuMA/dynein/dynactin exclusion from the equatorial cell membrane. The Ect2-based (Ect2/Cyk4/Mklp1) and NuMA-based (NuMA/dynein/dynactin) complexes occupy mutually exclusive membrane surfaces during anaphase. The equatorial membrane enrichment of Ect2-based complexes is es","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Dec","modification":"2025-04-04T10:41:52.499Z","creation":"2025-04-04T10:41:52.499Z"},"accession":"S-EPMC9539458","cross_references":{"pubmed":["36197340"],"doi":["10.1083/jcb.202203127"]}}