{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Huang Y"],"funding":["Deutsche Forschungsgemeinschaft"],"pagination":["e202206851"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9541201"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["61(34)"],"pubmed_abstract":["Naturally occurring α-pyrones with biological activities are mostly synthesised by polyketide synthases (PKSs) via iterative decarboxylative Claisen condensation steps. Remarkably, we found that some enzymes related to the fatty acid β-oxidation pathway in Escherichia coli, namely the CoA ligase FadD and the thiolases FadA and FadI, can synthesise styrylpyrones with phenylpropionic acids in vivo. The two thiolases directly utilise acetyl-CoA as an extender unit for carbon-chain elongation through a non-decarboxylative Claisen condensation, thus making the overall reaction more efficient in terms of carbon and energy consumption. Moreover, using a cell-free approach, different styrylpyrones were synthesised in vitro. Finally, targeted feeding experiments led to the detection of styrylpyrone"],"journal":["Angewandte Chemie (International ed. in English)"],"pubmed_title":["Intrinsic Ability of the β-Oxidation Pathway To Produce Bioactive Styrylpyrones."],"pmcid":["PMC9541201"],"funding_grant_id":["453246485","390713860"],"pubmed_authors":["Huang Y","Hoefgen S","Gherlone F","Valiante V"],"additional_accession":[]},"is_claimable":false,"name":"Intrinsic Ability of the β-Oxidation Pathway To Produce Bioactive Styrylpyrones.","description":"Naturally occurring α-pyrones with biological activities are mostly synthesised by polyketide synthases (PKSs) via iterative decarboxylative Claisen condensation steps. Remarkably, we found that some enzymes related to the fatty acid β-oxidation pathway in Escherichia coli, namely the CoA ligase FadD and the thiolases FadA and FadI, can synthesise styrylpyrones with phenylpropionic acids in vivo. The two thiolases directly utilise acetyl-CoA as an extender unit for carbon-chain elongation through a non-decarboxylative Claisen condensation, thus making the overall reaction more efficient in terms of carbon and energy consumption. Moreover, using a cell-free approach, different styrylpyrones were synthesised in vitro. Finally, targeted feeding experiments led to the detection of styrylpyrone","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Aug","modification":"2025-04-22T02:01:39.038Z","creation":"2025-04-05T20:11:59.469Z"},"accession":"S-EPMC9541201","cross_references":{"pubmed":["35726672"],"doi":["10.1002/anie.202206851"]}}