{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["234(4)"],"submitter":["Rudayni HA"],"funding":["National Health and Medical Research Council","Australian Research Council"],"pubmed_abstract":["<h4>Aim</h4>To develop a method for direct measurement of the fluorescent d-glucose analogue 2-NBDG transport across the plasma membrane of single skeletal muscle fibres and derive the theoretical framework for determining the kinetic parameters for d-glucose transport under basal conditions.<h4>Methods</h4>A novel method is described for measuring free 2-NBDG transport across plasma membrane of single rat muscle fibres at rest. The 2-NBDG uptake was >90% suppressed by 100 µM cytochalasin B in both fast-twitch and slow-twitch fibres, indicating that the 2-NBDG transport is GLUT-mediated. Fibres were identified as fast-twitch or slow-twitch based on the differential sensitivity of their contractile apparatus to Sr<sup>2+</sup> .<h4>Results</h4>The time course of 2-NBDG uptake in the presenc"],"journal":["Acta physiologica (Oxford, England)"],"pagination":["e13789"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9541404"],"repository":["biostudies-literature"],"pubmed_title":["Measurements of basal d-glucose transport through GLUT1 across the intact plasma membrane of isolated segments from single fast- and slow-twitch skeletal muscle fibres of rat."],"pmcid":["PMC9541404"],"pubmed_authors":["Rudayni HA","Stephenson G","Posterino GS"],"additional_accession":[]},"is_claimable":false,"name":"Measurements of basal d-glucose transport through GLUT1 across the intact plasma membrane of isolated segments from single fast- and slow-twitch skeletal muscle fibres of rat.","description":"<h4>Aim</h4>To develop a method for direct measurement of the fluorescent d-glucose analogue 2-NBDG transport across the plasma membrane of single skeletal muscle fibres and derive the theoretical framework for determining the kinetic parameters for d-glucose transport under basal conditions.<h4>Methods</h4>A novel method is described for measuring free 2-NBDG transport across plasma membrane of single rat muscle fibres at rest. The 2-NBDG uptake was >90% suppressed by 100 µM cytochalasin B in both fast-twitch and slow-twitch fibres, indicating that the 2-NBDG transport is GLUT-mediated. Fibres were identified as fast-twitch or slow-twitch based on the differential sensitivity of their contractile apparatus to Sr<sup>2+</sup> .<h4>Results</h4>The time course of 2-NBDG uptake in the presenc","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Apr","modification":"2025-04-19T04:48:12.658Z","creation":"2025-04-19T04:48:12.658Z"},"accession":"S-EPMC9541404","cross_references":{"pubmed":["35038771"],"doi":["10.1111/apha.13789"]}}