biostudies-literatureUnknown234(4)Rudayni HANational Health and Medical Research CouncilAustralian Research Council<h4>Aim</h4>To develop a method for direct measurement of the fluorescent d-glucose analogue 2-NBDG transport across the plasma membrane of single skeletal muscle fibres and derive the theoretical framework for determining the kinetic parameters for d-glucose transport under basal conditions.<h4>Methods</h4>A novel method is described for measuring free 2-NBDG transport across plasma membrane of single rat muscle fibres at rest. The 2-NBDG uptake was >90% suppressed by 100 µM cytochalasin B in both fast-twitch and slow-twitch fibres, indicating that the 2-NBDG transport is GLUT-mediated. Fibres were identified as fast-twitch or slow-twitch based on the differential sensitivity of their contractile apparatus to Sr²⁺ .<h4>Results</h4>The time course of 2-NBDG uptake in the presencActa physiologica (Oxford, England)e13789https://www.ebi.ac.uk/biostudies/studies/S-EPMC9541404biostudies-literatureMeasurements of basal d-glucose transport through GLUT1 across the intact plasma membrane of isolated segments from single fast- and slow-twitch skeletal muscle fibres of rat.PMC9541404Rudayni HAStephenson GPosterino GSfalseMeasurements of basal d-glucose transport through GLUT1 across the intact plasma membrane of isolated segments from single fast- and slow-twitch skeletal muscle fibres of rat.<h4>Aim</h4>To develop a method for direct measurement of the fluorescent d-glucose analogue 2-NBDG transport across the plasma membrane of single skeletal muscle fibres and derive the theoretical framework for determining the kinetic parameters for d-glucose transport under basal conditions.<h4>Methods</h4>A novel method is described for measuring free 2-NBDG transport across plasma membrane of single rat muscle fibres at rest. The 2-NBDG uptake was >90% suppressed by 100 µM cytochalasin B in both fast-twitch and slow-twitch fibres, indicating that the 2-NBDG transport is GLUT-mediated. Fibres were identified as fast-twitch or slow-twitch based on the differential sensitivity of their contractile apparatus to Sr²⁺ .<h4>Results</h4>The time course of 2-NBDG uptake in the presenc2022-01-01T00:00:00Z2022 Apr2025-04-19T04:48:12.658Z2025-04-19T04:48:12.658ZS-EPMC95414043503877110.1111/apha.13789