{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Ritonja JA"],"funding":["Cancer Research Society","Faculty of Health Sciences, Queen’s University"],"pagination":["1259-1268"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9542576"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(10)"],"pubmed_abstract":["Night shift work is associated with increased breast cancer risk, but the molecular mechanisms are not well-understood. The objective of this study was to explore the relationship between night shift work parameters (current status, duration/years, and intensity) and methylation in circadian genes as a potential mechanism underlying the carcinogenic effects of night shift work. A cross-sectional study was conducted among 74 female healthcare employees (n = 38 day workers, n = 36 night shift workers). The Illumina Infinium MethylationEPIC beadchip was applied to DNA extracted from blood samples to measure methylation using a candidate gene approach at 1150 CpG loci across 22 circadian genes. Linear regression models were used to examine the association between night shift work parameters and continuous methylation measurements (β-values) for each CpG site. The false-discovery rate (q = 0.2) was used to account for multiple comparisons. Compared to day workers, current night shift workers demonstrated hypermethylation in the 5'UTR region of <i>CSNK1E</i> (q = 0.15). Individuals that worked night shifts for ≥10 years exhibited hypomethylation in the gene body of <i>NR1D1</i> (q = 0.08) compared to those that worked <10 years. Hypermethylation in the gene body of <i>ARNTL</i> was also apparent in those who worked ≥3 consecutive night shifts a week (q = 0.18). These findings suggest that night shift work is associated with differential methylation in core circadian genes, including <i>CSNK1E, NR1D1</i> and <i>ARNTL</i>. Future, larger-scale studies with long-term follow-up and detailed night shift work assessment are needed to confirm and expand on these findings."],"journal":["Epigenetics"],"pubmed_title":["Exploring the impact of night shift work on methylation of circadian genes."],"pmcid":["PMC9542576"],"funding_grant_id":["23218","Garfield Kelly Cardiovascular Research &amp; Development Fund (6026402)"],"pubmed_authors":["Flaten L","Duan QL","Aronson KJ","Topouza DG","Tranmer JE","Bhatti P","Durocher F","Ritonja JA"],"additional_accession":[]},"is_claimable":false,"name":"Exploring the impact of night shift work on methylation of circadian genes.","description":"Night shift work is associated with increased breast cancer risk, but the molecular mechanisms are not well-understood. The objective of this study was to explore the relationship between night shift work parameters (current status, duration/years, and intensity) and methylation in circadian genes as a potential mechanism underlying the carcinogenic effects of night shift work. A cross-sectional study was conducted among 74 female healthcare employees (n = 38 day workers, n = 36 night shift workers). The Illumina Infinium MethylationEPIC beadchip was applied to DNA extracted from blood samples to measure methylation using a candidate gene approach at 1150 CpG loci across 22 circadian genes. Linear regression models were used to examine the association between night shift work parameters and continuous methylation measurements (β-values) for each CpG site. The false-discovery rate (q = 0.2) was used to account for multiple comparisons. Compared to day workers, current night shift workers demonstrated hypermethylation in the 5'UTR region of <i>CSNK1E</i> (q = 0.15). Individuals that worked night shifts for ≥10 years exhibited hypomethylation in the gene body of <i>NR1D1</i> (q = 0.08) compared to those that worked <10 years. Hypermethylation in the gene body of <i>ARNTL</i> was also apparent in those who worked ≥3 consecutive night shifts a week (q = 0.18). These findings suggest that night shift work is associated with differential methylation in core circadian genes, including <i>CSNK1E, NR1D1</i> and <i>ARNTL</i>. Future, larger-scale studies with long-term follow-up and detailed night shift work assessment are needed to confirm and expand on these findings.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-25T21:02:42.835Z","creation":"2025-04-06T08:33:26.612Z"},"accession":"S-EPMC9542576","cross_references":{"pubmed":["34825628"],"doi":["10.1080/15592294.2021.2009997"]}}