biostudies-literatureXiao LNatural Science Foundation of Zhejiang ProvincePostdoctoral Research Foundation of ChinaNational Natural Science Foundation of China1205-1218https://www.ebi.ac.uk/biostudies/studies/S-EPMC9543047biostudies-literatureUnknown17(10)Preimplantation development is critical for reproductive successes in mammals. Thus, it is important to understand how preimplantation embryogenesis is regulated. As a key event of preimplantation development, epigenetic reprogramming has been widely studied, yet how epigenetic complexes regulate preimplantation development remains largely unknown. Retinoblastoma binding protein 4 (RBBP4) and 7 (RBBP7) are integral components of epigenetic complexes including SIN3A, NuRD, and CoREST. Here, we demonstrate that double knockdown of <i>Rbbp4</i> and <i>7</i>, but not individually, causes embryonic lethality during the morula-to-blastocyst transition. Mechanistically, depletion of RBBP4 and 7 results in dysregulation of genes related to cell cycle, lineage development, and regulation of transcrEpigeneticsOverlapping functions of RBBP4 and RBBP7 in regulating cell proliferation and histone H3.3 deposition during mouse preimplantation development.PMC9543047No. 2020M671742Xiao LZhang KWang SLuo LZhao PDang YHu BfalseOverlapping functions of RBBP4 and RBBP7 in regulating cell proliferation and histone H3.3 deposition during mouse preimplantation development.Preimplantation development is critical for reproductive successes in mammals. Thus, it is important to understand how preimplantation embryogenesis is regulated. As a key event of preimplantation development, epigenetic reprogramming has been widely studied, yet how epigenetic complexes regulate preimplantation development remains largely unknown. Retinoblastoma binding protein 4 (RBBP4) and 7 (RBBP7) are integral components of epigenetic complexes including SIN3A, NuRD, and CoREST. Here, we demonstrate that double knockdown of <i>Rbbp4</i> and <i>7</i>, but not individually, causes embryonic lethality during the morula-to-blastocyst transition. Mechanistically, depletion of RBBP4 and 7 results in dysregulation of genes related to cell cycle, lineage development, and regulation of transcr2022-01-01T00:00:00Z2022 Oct2025-04-04T19:27:30.482Z2025-04-04T19:27:30.482ZS-EPMC95430473470911310.1080/15592294.2021.1999006