{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Natalini A"],"funding":["Consiglio Nazionale delle Ricerche","Italian Minister of Research and University (MIUR)","The Francis Crick Institute"],"pagination":["1171-1175"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9543383"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["99(12)"],"pubmed_abstract":["A multicolor flow cytometry panel was designed and optimized to define the following nine mouse T cell subsets: Treg (CD3+ CD4+ CD8- FoxP3+ ), CD4+ T naïve (CD3+ CD4+ CD8- FoxP3- CD44int/low CD62L+ ), CD4+ T central memory (CD3+ CD4+ CD8- FoxP3- CD44high CD62L+ ), CD4+ T effector memory (CD3+ CD4+ CD8- FoxP3- CD44high CD62L- ), CD4+ T EMRA (CD3+ CD4+ CD8- FoxP3- CD44int/low CD62L- ), CD8+ T naïve (CD3+ CD8+ CD4- CD44int/low CD62L+ ), CD8+ T central memory (CD3+ CD8+ CD4- CD44high CD62L+ ), CD8+ T effector memory (CD3+ CD8+ CD4- CD44high CD62L- ), and CD8+ T EMRA (CD3+ CD8+ CD4- CD44int/low CD62L- ). In each T cell subset, a dual staining for Ki-67 expression and DNA content was employed to distinguish the following cell cycle phases: G0 (Ki67- , with 2n DNA), G1 (Ki67+ , with 2n DNA), and S-G2 /M (Ki67+ , with 2n < DNA ≤ 4n). This panel was established for the analysis of mouse (C57BL/6J) spleen."],"journal":["Cytometry. Part A : the journal of the International Society for Analytical Cytology"],"pubmed_title":["OMIP-079: Cell cycle of CD4+ and CD8+ naive/memory T cell subsets, and of Treg cells from mouse spleen."],"pmcid":["PMC9543383"],"funding_grant_id":["STM‐2019","10093","2017K55HLC","STM-2019","10729","10794"],"pubmed_authors":["Natalini A","Di Rosa F","Santoni A","Simonetti S","Hayday A","Antonangeli F","Munoz-Ruiz M","Peruzzi G","Favaretto G"],"additional_accession":[]},"is_claimable":false,"name":"OMIP-079: Cell cycle of CD4+ and CD8+ naive/memory T cell subsets, and of Treg cells from mouse spleen.","description":"A multicolor flow cytometry panel was designed and optimized to define the following nine mouse T cell subsets: Treg (CD3+ CD4+ CD8- FoxP3+ ), CD4+ T naïve (CD3+ CD4+ CD8- FoxP3- CD44int/low CD62L+ ), CD4+ T central memory (CD3+ CD4+ CD8- FoxP3- CD44high CD62L+ ), CD4+ T effector memory (CD3+ CD4+ CD8- FoxP3- CD44high CD62L- ), CD4+ T EMRA (CD3+ CD4+ CD8- FoxP3- CD44int/low CD62L- ), CD8+ T naïve (CD3+ CD8+ CD4- CD44int/low CD62L+ ), CD8+ T central memory (CD3+ CD8+ CD4- CD44high CD62L+ ), CD8+ T effector memory (CD3+ CD8+ CD4- CD44high CD62L- ), and CD8+ T EMRA (CD3+ CD8+ CD4- CD44int/low CD62L- ). In each T cell subset, a dual staining for Ki-67 expression and DNA content was employed to distinguish the following cell cycle phases: G0 (Ki67- , with 2n DNA), G1 (Ki67+ , with 2n DNA), and S-G2 /M (Ki67+ , with 2n < DNA ≤ 4n). This panel was established for the analysis of mouse (C57BL/6J) spleen.","dates":{"release":"2021-01-01T00:00:00Z","publication":"2021 Dec","modification":"2025-04-05T20:11:06.586Z","creation":"2025-04-05T20:11:06.586Z"},"accession":"S-EPMC9543383","cross_references":{"pubmed":["34668313"],"doi":["10.1002/cyto.a.24509"]}}