biostudies-literatureNatalini AConsiglio Nazionale delle RicercheItalian Minister of Research and University (MIUR)The Francis Crick Institute1171-1175https://www.ebi.ac.uk/biostudies/studies/S-EPMC9543383biostudies-literatureUnknown99(12)A multicolor flow cytometry panel was designed and optimized to define the following nine mouse T cell subsets: Treg (CD3⁺ CD4⁺ CD8^- FoxP3⁺ ), CD4⁺ T naïve (CD3⁺ CD4⁺ CD8^- FoxP3^- CD44^int/low CD62L⁺ ), CD4⁺ T central memory (CD3⁺ CD4⁺ CD8^- FoxP3^- CD44^high CD62L⁺ ), CD4⁺ T effector memory (CD3⁺ CD4⁺ CD8^- FoxP3^- CD44^high CD62L^- ), CD4⁺ T EMRA (CD3⁺ CD4⁺ CD8^- FoxP3^- CD44^int/low CD62L^- ), CD8⁺ T naïve (CD3⁺ CD8⁺ CD4^- CD44^int/low CD62L⁺ ), CD8⁺ T central memory (CD3⁺ CD8⁺ CD4^- CD44^high CD62L⁺ ), CD8⁺ T effector memory (CD3⁺ CD8⁺ CD4^- CD44^high CD62L^- ), and CD8⁺ T EMRA (CD3⁺ CD8⁺ CD4^- CD44^int/low CD62L^- ). In each T cell subset, a dual staining for Ki-67 expression and DNA content was employed to distinguish the following cell cycle phases: G₀ (Ki67^- , with 2n DNA), G₁ (Ki67⁺ , with 2n DNA), and S-G₂ /M (Ki67⁺ , with 2n < DNA ≤ 4n). This panel was established for the analysis of mouse (C57BL/6J) spleen.Cytometry. Part A : the journal of the International Society for Analytical CytologyOMIP-079: Cell cycle of CD4⁺ and CD8⁺ naive/memory T cell subsets, and of Treg cells from mouse spleen.PMC9543383STM‐2019100932017K55HLCSTM-20191072910794Natalini ADi Rosa FSantoni ASimonetti SHayday AAntonangeli FMunoz-Ruiz MPeruzzi GFavaretto GfalseOMIP-079: Cell cycle of CD4⁺ and CD8⁺ naive/memory T cell subsets, and of Treg cells from mouse spleen.A multicolor flow cytometry panel was designed and optimized to define the following nine mouse T cell subsets: Treg (CD3⁺ CD4⁺ CD8^- FoxP3⁺ ), CD4⁺ T naïve (CD3⁺ CD4⁺ CD8^- FoxP3^- CD44^int/low CD62L⁺ ), CD4⁺ T central memory (CD3⁺ CD4⁺ CD8^- FoxP3^- CD44^high CD62L⁺ ), CD4⁺ T effector memory (CD3⁺ CD4⁺ CD8^- FoxP3^- CD44^high CD62L^- ), CD4⁺ T EMRA (CD3⁺ CD4⁺ CD8^- FoxP3^- CD44^int/low CD62L^- ), CD8⁺ T naïve (CD3⁺ CD8⁺ CD4^- CD44^int/low CD62L⁺ ), CD8⁺ T central memory (CD3⁺ CD8⁺ CD4^- CD44^high CD62L⁺ ), CD8⁺ T effector memory (CD3⁺ CD8⁺ CD4^- CD44^high CD62L^- ), and CD8⁺ T EMRA (CD3⁺ CD8⁺ CD4^- CD44^int/low CD62L^- ). In each T cell subset, a dual staining for Ki-67 expression and DNA content was employed to distinguish the following cell cycle phases: G₀ (Ki67^- , with 2n DNA), G₁ (Ki67⁺ , with 2n DNA), and S-G₂ /M (Ki67⁺ , with 2n < DNA ≤ 4n). This panel was established for the analysis of mouse (C57BL/6J) spleen.2021-01-01T00:00:00Z2021 Dec2025-04-05T20:11:06.586Z2025-04-05T20:11:06.586ZS-EPMC95433833466831310.1002/cyto.a.24509