biostudies-literatureUnknown112(2)You RThe stromal niche plays a pivotal role in AML chemoresistance and energy metabolism reprogramming is a hallmark of a tumor. 5'-Adenosine monophosphate-activated protein kinase (AMPK) is an important energy sensor suppressing mammalian target of rapamycin complex 1 (mTORC1) activity. However, the role of AMPK-mTORC1 pathway on connecting AML cell energy metabolism reprogramming and chemoresistance induced by the bone marrow microenvironment (BMM) is not defined. Here, with a co-culture system that simulates the interaction between BMM and AML cells, it is shown that stromal contact led to a decreased sensitivity to chemotherapy accompanied by an increase of oxidative phosphorylation (OXPHOS) activity and mitochondrial ATP synthesis in AML cells. The increased OXPHOS activity and excessive AJournal of leukocyte biology299-311https://www.ebi.ac.uk/biostudies/studies/S-EPMC9544716biostudies-literatureBone marrow microenvironment drives AML cell OXPHOS addiction and AMPK inhibition to resist chemotherapy.PMC9544716Liu JPan ZChen PWang BLi DFeng XHuang HHou DYou RWang XKang LXiao QfalseBone marrow microenvironment drives AML cell OXPHOS addiction and AMPK inhibition to resist chemotherapy.The stromal niche plays a pivotal role in AML chemoresistance and energy metabolism reprogramming is a hallmark of a tumor. 5'-Adenosine monophosphate-activated protein kinase (AMPK) is an important energy sensor suppressing mammalian target of rapamycin complex 1 (mTORC1) activity. However, the role of AMPK-mTORC1 pathway on connecting AML cell energy metabolism reprogramming and chemoresistance induced by the bone marrow microenvironment (BMM) is not defined. Here, with a co-culture system that simulates the interaction between BMM and AML cells, it is shown that stromal contact led to a decreased sensitivity to chemotherapy accompanied by an increase of oxidative phosphorylation (OXPHOS) activity and mitochondrial ATP synthesis in AML cells. The increased OXPHOS activity and excessive A2022-01-01T00:00:00Z2022 Aug2025-04-22T02:01:16.341Z2025-04-05T20:11:53.966ZS-EPMC95447163492774310.1002/JLB.6A0821-409RR