biostudies-literatureFalco MInstituto de Salud Carlos III107-118https://www.ebi.ac.uk/biostudies/studies/S-EPMC9544867biostudies-literatureUnknown100(2)Killer immunoglobulin-like receptor (KIR) genes code for a family of inhibitory and activating receptors, finely tuning NK cell function. Numerous studies reported the relevance of KIR allelic polymorphism on KIR expression, ligand affinity, and strength in signal transduction. Although KIR variability, including gene copy number and allelic polymorphism, in combination with HLA class I polymorphism, impacts both KIR expression and NK cell education, only a precise phenotypic analysis can define the size of the different KIR^pos NK cell subsets. In this context, reagents recognizing a limited number of KIRs is essential. In this study, we have characterized the specificity of an anti-KIR mAb termed HP-DM1. Testing its binding to HEK-293T cells transfected with plasmids coding forHLAEpitope characterization of a monoclonal antibody that selectively recognizes KIR2DL1 allotypes.PMC9544867AIRC 5 × 1000 2018 id. 21147FIS00/0181H2020‐MSCA‐ITN‐2017‐765104‐MATURE‐NKFalco MMuntasell ALopez-Botet MAlicata CBottino CPende DMoretta LMeazza RCanevali PfalseEpitope characterization of a monoclonal antibody that selectively recognizes KIR2DL1 allotypes.Killer immunoglobulin-like receptor (KIR) genes code for a family of inhibitory and activating receptors, finely tuning NK cell function. Numerous studies reported the relevance of KIR allelic polymorphism on KIR expression, ligand affinity, and strength in signal transduction. Although KIR variability, including gene copy number and allelic polymorphism, in combination with HLA class I polymorphism, impacts both KIR expression and NK cell education, only a precise phenotypic analysis can define the size of the different KIR^pos NK cell subsets. In this context, reagents recognizing a limited number of KIRs is essential. In this study, we have characterized the specificity of an anti-KIR mAb termed HP-DM1. Testing its binding to HEK-293T cells transfected with plasmids coding for2022-01-01T00:00:00Z2022 Aug2026-05-31T16:08:28.166Z2025-02-19T01:28:08.78ZS-EPMC95448673541163410.1111/tan.14630