biostudies-literatureUnknown13Yang XThe coronavirus disease 2019 pandemic has caused more than 532 million infections and 6.3 million deaths to date. The reactive and neutralizing fully human antibodies of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are effective detection tools and therapeutic measures. During SARS-CoV-2 infection, a large number of SARS-CoV-2 reactive and neutralizing antibodies will be produced. Most SARS-CoV-2 reactive and neutralizing fully human antibodies are isolated from human and frequently encoded by convergent heavy-chain variable genes. However, SARS-CoV-2 viruses can mutate rapidly during replication and the resistant variants of neutralizing antibodies easily survive and evade the immune response, especially in the face of such focused antibody responses in humans. Therefore, Frontiers in immunology992787https://www.ebi.ac.uk/biostudies/studies/S-EPMC9545174biostudies-literatureDiscovery and characterization of SARS-CoV-2 reactive and neutralizing antibodies from humanized CAMouse^HG mice through rapid hybridoma screening and high-throughput single-cell V(D)J sequencing.PMC9545174Xia XWu MHan QLang QHuang NGe LChi HBi JZhao YYang XLiu XFeng NLi YLiang HWang TGao YWang XWang ZYang SfalseDiscovery and characterization of SARS-CoV-2 reactive and neutralizing antibodies from humanized CAMouse^HG mice through rapid hybridoma screening and high-throughput single-cell V(D)J sequencing.The coronavirus disease 2019 pandemic has caused more than 532 million infections and 6.3 million deaths to date. The reactive and neutralizing fully human antibodies of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) are effective detection tools and therapeutic measures. During SARS-CoV-2 infection, a large number of SARS-CoV-2 reactive and neutralizing antibodies will be produced. Most SARS-CoV-2 reactive and neutralizing fully human antibodies are isolated from human and frequently encoded by convergent heavy-chain variable genes. However, SARS-CoV-2 viruses can mutate rapidly during replication and the resistant variants of neutralizing antibodies easily survive and evade the immune response, especially in the face of such focused antibody responses in humans. Therefore, 2022-01-01T00:00:00Z20222026-04-08T11:33:47.327Z2024-11-12T02:40:42.921ZS-EPMC95451743621141010.3389/fimmu.2022.992787