{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Nilsson T"],"funding":["Vetenskapsrådet","Västra Götalandsregionen","Barncancerfonden","Cancerfonden"],"pagination":["770-782"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9545334"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["151(5)"],"pubmed_abstract":["Acute myeloid leukemia (AML) results from aberrant hematopoietic processes and these changes are frequently initiated by chromosomal translocations. One particular subtype, AML with translocation t(7;12)(q36;p13), is found in children diagnosed before 2 years of age. The mechanisms for leukemogenesis induced by t(7;12) is not understood, in part because of the lack of efficient methods to reconstruct the leukemia-associated genetic aberration with correct genomic architecture and regulatory elements. We therefore created induced pluripotent stem cell (iPSC) lines that carry the translocation t(7;12) using CRISPR/Cas9. These t(7;12) iPSC showed propensity to differentiate into all three germ layers, confirming retained stem cell properties. The potential for differentiation into hematopoiet"],"journal":["International journal of cancer"],"pubmed_title":["An induced pluripotent stem cell t(7;12)(q36;p13) acute myeloid leukemia model shows high expression of MNX1 and a block in differentiation of the erythroid and megakaryocytic lineages."],"pmcid":["PMC9545334"],"funding_grant_id":["ALFGBG‐431881","2018‐05973","PR2014‐0125","PR2019‐0013","TJ2019‐0053","CAN2017/461"],"pubmed_authors":["Abrahamsson J","Asp J","Li S","Fogelstrand L","Ostlund A","Nilsson T","Palmqvist L","Waraky A","Staffas A"],"additional_accession":[]},"is_claimable":false,"name":"An induced pluripotent stem cell t(7;12)(q36;p13) acute myeloid leukemia model shows high expression of MNX1 and a block in differentiation of the erythroid and megakaryocytic lineages.","description":"Acute myeloid leukemia (AML) results from aberrant hematopoietic processes and these changes are frequently initiated by chromosomal translocations. One particular subtype, AML with translocation t(7;12)(q36;p13), is found in children diagnosed before 2 years of age. The mechanisms for leukemogenesis induced by t(7;12) is not understood, in part because of the lack of efficient methods to reconstruct the leukemia-associated genetic aberration with correct genomic architecture and regulatory elements. We therefore created induced pluripotent stem cell (iPSC) lines that carry the translocation t(7;12) using CRISPR/Cas9. These t(7;12) iPSC showed propensity to differentiate into all three germ layers, confirming retained stem cell properties. The potential for differentiation into hematopoiet","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Sep","modification":"2026-04-08T11:50:11.245Z","creation":"2025-02-19T01:02:58.144Z"},"accession":"S-EPMC9545334","cross_references":{"pubmed":["35583991"],"doi":["10.1002/ijc.34122"]}}