biostudies-literatureUnknown101(2)Carsetti RThe B cell compartment provides innate and adaptive immune defenses against pathogens. Different B cell subsets, reflecting the maturation stages of B cells, have noninterchangeable functions and roles in innate and adaptive immune responses. In this review, we provide an overview of the B cell subsets present in peripheral blood of healthy individuals. A specific gating strategy is also described to clearly and univocally identify B cell subsets based on the their phenotypic traits by flow cytometric analysis.Cytometry. Part A : the journal of the International Society for Analytical Cytology131-139https://www.ebi.ac.uk/biostudies/studies/S-EPMC9546334biostudies-literatureComprehensive phenotyping of human peripheral blood B lymphocytes in healthy conditions.PMC9546334Terreri SCorrente FFernandez Salinas APiano Mortari EConti MGCapponi CAlbano CCarsetti RfalseComprehensive phenotyping of human peripheral blood B lymphocytes in healthy conditions.The B cell compartment provides innate and adaptive immune defenses against pathogens. Different B cell subsets, reflecting the maturation stages of B cells, have noninterchangeable functions and roles in innate and adaptive immune responses. In this review, we provide an overview of the B cell subsets present in peripheral blood of healthy individuals. A specific gating strategy is also described to clearly and univocally identify B cell subsets based on the their phenotypic traits by flow cytometric analysis.2022-01-01T00:00:00Z2022 Feb2025-04-22T12:09:20.327Z2025-04-06T00:12:33.467ZS-EPMC95463343466439710.1002/cyto.a.24507