biostudies-literatureUnknown13Ye X<h4>Background</h4>Gasdermin D (GSDMD) plays an essential role in the pathway of pyroptosis. However, whether GSDMD participates in myocardial ischaemia/reperfusion injury (MI/RI) remains poorly understood.<h4>Methods</h4>Serum levels of GSDMD and IL-18 in ST-segment elevation myocardial infarction (STEMI) patients were measured by ELISA. The expression of GSDMD and GSDMD N-terminal (GSDMD-NT) <i>in vivo</i> and <i>in vitro</i> was assessed by western blot and immunofluorescence staining. GSDMD^-/- mice and wild type (WT) mice were induced MI/RI, followed by cardiac ultrasound and histological analysis.<h4>Results</h4>Clinically, patients suffering from STEMI after percutaneous coronary intervention (PCI) exhibited higher levels of GSDMD and IL-18 than that in the controls. <i>InFrontiers in immunology893914https://www.ebi.ac.uk/biostudies/studies/S-EPMC9546776biostudies-literatureGSDMD contributes to myocardial reperfusion injury by regulating pyroptosis.PMC9546776Wu ZZhang YHu WXu CJu DYe XLuan JZhang PfalseGSDMD contributes to myocardial reperfusion injury by regulating pyroptosis.<h4>Background</h4>Gasdermin D (GSDMD) plays an essential role in the pathway of pyroptosis. However, whether GSDMD participates in myocardial ischaemia/reperfusion injury (MI/RI) remains poorly understood.<h4>Methods</h4>Serum levels of GSDMD and IL-18 in ST-segment elevation myocardial infarction (STEMI) patients were measured by ELISA. The expression of GSDMD and GSDMD N-terminal (GSDMD-NT) <i>in vivo</i> and <i>in vitro</i> was assessed by western blot and immunofluorescence staining. GSDMD^-/- mice and wild type (WT) mice were induced MI/RI, followed by cardiac ultrasound and histological analysis.<h4>Results</h4>Clinically, patients suffering from STEMI after percutaneous coronary intervention (PCI) exhibited higher levels of GSDMD and IL-18 than that in the controls. <i>In2022-01-01T00:00:00Z20222026-05-31T16:06:56.073Z2025-02-19T01:28:03.415ZS-EPMC95467763621754310.3389/fimmu.2022.893914