biostudies-literatureUnknown5(1)Zhang SHuman endometrium undergoes cycles of regeneration in women of reproductive age. The endometrial mesenchymal stromal/stem cells (eMSC) contribute to this process. Notch signaling is essential for homeostasis of somatic stem cells. However, its role in eMSC remains unclear. We show with gain- and loss-of-function experiments that activation of Notch signaling promotes eMSC maintenance, while inhibition induces opposite effect. The activation of Notch signaling better maintains eMSC in a quiescent state. However, these quiescent eMSC can re-enter the cell cycle depending on the Notch and Wnt activities in the microenvironment, suggesting a crosstalk between the two signaling pathways. We further show that the Notch signaling is involved in endometrial remodeling event in a mouse menstrual-liCommunications biology1064https://www.ebi.ac.uk/biostudies/studies/S-EPMC9547015biostudies-literatureThe role of Notch signaling in endometrial mesenchymal stromal/stem-like cells maintenance.PMC9547015Yeung WSBChan RWSNg EHYZhang SfalseThe role of Notch signaling in endometrial mesenchymal stromal/stem-like cells maintenance.Human endometrium undergoes cycles of regeneration in women of reproductive age. The endometrial mesenchymal stromal/stem cells (eMSC) contribute to this process. Notch signaling is essential for homeostasis of somatic stem cells. However, its role in eMSC remains unclear. We show with gain- and loss-of-function experiments that activation of Notch signaling promotes eMSC maintenance, while inhibition induces opposite effect. The activation of Notch signaling better maintains eMSC in a quiescent state. However, these quiescent eMSC can re-enter the cell cycle depending on the Notch and Wnt activities in the microenvironment, suggesting a crosstalk between the two signaling pathways. We further show that the Notch signaling is involved in endometrial remodeling event in a mouse menstrual-li2022-01-01T00:00:00Z2022 Oct2025-04-25T17:36:35.686Z2025-04-06T05:23:09.276ZS-EPMC95470153620760510.1038/s42003-022-04044-x