biostudies-literatureUnknown13(3)Tavarideh FTo search endophytic bacteria diversity and evaluate their antibacterial activity, healthy medicinal plant of <i>Scrophularia striata</i> was chosen in this study. One hundred endophytic bacteria were isolated from surface-sterilized tissues (root, stem and leaf) of <i>S. striata</i>. Using sequence analysis targeting 16S rRNA gene, eight genera, including <i>Agrococcus</i>, <i>Arthrobacter</i>, <i>Bacillus</i>, <i>Chryseobacterium</i>, <i>Delftia</i>, <i>Kocuria</i>, <i>Pseudomonas</i> and <i>Sphingomonas</i> were identified. Antibacterial activity of endophytic bacteria was examined against some test bacteria, employing agar well diffusion method. Out of 31 endophytic bacterial isolates, 24(77.42%) isolates showed significant antimicrobial activity against <i>Bacillus cereus</i>, 17(54.8Veterinary research forum : an international quarterly journal409-415https://www.ebi.ac.uk/biostudies/studies/S-EPMC9548236biostudies-literatureDiversity and antibacterial activity of endophytic bacteria associated with medicinal plant, <i>Scrophularia striata</i>.PMC9548236Tavarideh FNemati MPourahmad FfalseDiversity and antibacterial activity of endophytic bacteria associated with medicinal plant, <i>Scrophularia striata</i>.To search endophytic bacteria diversity and evaluate their antibacterial activity, healthy medicinal plant of <i>Scrophularia striata</i> was chosen in this study. One hundred endophytic bacteria were isolated from surface-sterilized tissues (root, stem and leaf) of <i>S. striata</i>. Using sequence analysis targeting 16S rRNA gene, eight genera, including <i>Agrococcus</i>, <i>Arthrobacter</i>, <i>Bacillus</i>, <i>Chryseobacterium</i>, <i>Delftia</i>, <i>Kocuria</i>, <i>Pseudomonas</i> and <i>Sphingomonas</i> were identified. Antibacterial activity of endophytic bacteria was examined against some test bacteria, employing agar well diffusion method. Out of 31 endophytic bacterial isolates, 24(77.42%) isolates showed significant antimicrobial activity against <i>Bacillus cereus</i>, 17(54.82022-01-01T00:00:00Z2022 Sep2025-04-04T22:33:46.243Z2025-02-19T03:24:07.315ZS-EPMC95482363632030710.30466/vrf.2021.529714.3174