biostudies-literatureUnknown7(1)Greenlee JDWhile subcutaneous tumor models remain the standard for studying drug efficacy <i>in vivo</i>, these tumors rarely metastasize and lack physiological relevance due to differences in the tumor microenvironment, vascularization, immune landscape, and physiological cues associated with the organ of interest. Orthotopic tumors, grown from the organ corresponding with the cancer type, provide a more translational approach to study disease progression and drug efficacy. Utilization of a syngeneic mouse model allows for a complete immune landscape, key for adaptive immunotherapy studies. MC38 and CT26 cells are commonly used murine colorectal cancer cell lines with clinically relevant mutations. While CT26 cells have been orthotopically implanted with high fidelity, successful engraftment of orthBiology methods & protocolsbpac024https://www.ebi.ac.uk/biostudies/studies/S-EPMC9550231biostudies-literatureA syngeneic MC38 orthotopic mouse model of colorectal cancer metastasis.PMC9550231King MRGreenlee JDfalseA syngeneic MC38 orthotopic mouse model of colorectal cancer metastasis.While subcutaneous tumor models remain the standard for studying drug efficacy <i>in vivo</i>, these tumors rarely metastasize and lack physiological relevance due to differences in the tumor microenvironment, vascularization, immune landscape, and physiological cues associated with the organ of interest. Orthotopic tumors, grown from the organ corresponding with the cancer type, provide a more translational approach to study disease progression and drug efficacy. Utilization of a syngeneic mouse model allows for a complete immune landscape, key for adaptive immunotherapy studies. MC38 and CT26 cells are commonly used murine colorectal cancer cell lines with clinically relevant mutations. While CT26 cells have been orthotopically implanted with high fidelity, successful engraftment of orth2022-01-01T00:00:00Z20222025-04-05T12:18:52.994Z2025-04-05T12:18:52.994ZS-EPMC95502313622559510.1093/biomethods/bpac024