{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["2022"],"submitter":["Sun Q"],"pubmed_abstract":["<h4>Purpose</h4>The purpose of this study was to explore the role of the lncRNA MNX1-AS1 and its related downstream signaling pathways in colorectal adenocarcinoma (COAD).<h4>Methods</h4>COAD tissues and cells were prepared and treated with sh-MNX1-AS1, pcDNA-MNX1-AS1, sh-PPFIA4, LY29004, and their controls. CCK8 and colony formation assays were undertaken for evaluating cell proliferation. Tumor cell migratory ability was detected by transwell assay. Apoptosis detection was processed by YO-PRO-1/PI Staining. The regulated relationship between lncRNA MNX1-AS1 and PPFIA4 was confirmed by RIP-ChIP assay. Q-PCR was applied to detect genes related to tumor cell stemness, proliferation, migration, and apoptosis in each group. Finally, a xenograft tumor model was constructed to verify the result"],"journal":["Journal of oncology"],"pagination":["8303409"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9550508"],"repository":["biostudies-literature"],"pubmed_title":["Overexpression of LncRNA MNX1-AS1/PPFIA4 Activates AKT/HIF-1<i>α</i> Signal Pathway to Promote Stemness of Colorectal Adenocarcinoma Cells."],"pmcid":["PMC9550508"],"pubmed_authors":["Zhu J","Sun Q","Hu H","Zhao W","Gui Z","Zhao Z","Xu W","Gao C"],"additional_accession":[]},"is_claimable":false,"name":"Overexpression of LncRNA MNX1-AS1/PPFIA4 Activates AKT/HIF-1<i>α</i> Signal Pathway to Promote Stemness of Colorectal Adenocarcinoma Cells.","description":"<h4>Purpose</h4>The purpose of this study was to explore the role of the lncRNA MNX1-AS1 and its related downstream signaling pathways in colorectal adenocarcinoma (COAD).<h4>Methods</h4>COAD tissues and cells were prepared and treated with sh-MNX1-AS1, pcDNA-MNX1-AS1, sh-PPFIA4, LY29004, and their controls. CCK8 and colony formation assays were undertaken for evaluating cell proliferation. Tumor cell migratory ability was detected by transwell assay. Apoptosis detection was processed by YO-PRO-1/PI Staining. The regulated relationship between lncRNA MNX1-AS1 and PPFIA4 was confirmed by RIP-ChIP assay. Q-PCR was applied to detect genes related to tumor cell stemness, proliferation, migration, and apoptosis in each group. Finally, a xenograft tumor model was constructed to verify the result","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2025-04-21T16:53:17.931Z","creation":"2025-04-21T16:53:17.931Z"},"accession":"S-EPMC9550508","cross_references":{"pubmed":["36226248"],"doi":["10.1155/2022/8303409"]}}