biostudies-literatureUnknown2022Sun Q<h4>Purpose</h4>The purpose of this study was to explore the role of the lncRNA MNX1-AS1 and its related downstream signaling pathways in colorectal adenocarcinoma (COAD).<h4>Methods</h4>COAD tissues and cells were prepared and treated with sh-MNX1-AS1, pcDNA-MNX1-AS1, sh-PPFIA4, LY29004, and their controls. CCK8 and colony formation assays were undertaken for evaluating cell proliferation. Tumor cell migratory ability was detected by transwell assay. Apoptosis detection was processed by YO-PRO-1/PI Staining. The regulated relationship between lncRNA MNX1-AS1 and PPFIA4 was confirmed by RIP-ChIP assay. Q-PCR was applied to detect genes related to tumor cell stemness, proliferation, migration, and apoptosis in each group. Finally, a xenograft tumor model was constructed to verify the resultJournal of oncology8303409https://www.ebi.ac.uk/biostudies/studies/S-EPMC9550508biostudies-literatureOverexpression of LncRNA MNX1-AS1/PPFIA4 Activates AKT/HIF-1<i>α</i> Signal Pathway to Promote Stemness of Colorectal Adenocarcinoma Cells.PMC9550508Zhu JSun QHu HZhao WGui ZZhao ZXu WGao CfalseOverexpression of LncRNA MNX1-AS1/PPFIA4 Activates AKT/HIF-1<i>α</i> Signal Pathway to Promote Stemness of Colorectal Adenocarcinoma Cells.<h4>Purpose</h4>The purpose of this study was to explore the role of the lncRNA MNX1-AS1 and its related downstream signaling pathways in colorectal adenocarcinoma (COAD).<h4>Methods</h4>COAD tissues and cells were prepared and treated with sh-MNX1-AS1, pcDNA-MNX1-AS1, sh-PPFIA4, LY29004, and their controls. CCK8 and colony formation assays were undertaken for evaluating cell proliferation. Tumor cell migratory ability was detected by transwell assay. Apoptosis detection was processed by YO-PRO-1/PI Staining. The regulated relationship between lncRNA MNX1-AS1 and PPFIA4 was confirmed by RIP-ChIP assay. Q-PCR was applied to detect genes related to tumor cell stemness, proliferation, migration, and apoptosis in each group. Finally, a xenograft tumor model was constructed to verify the result2022-01-01T00:00:00Z20222025-04-21T16:53:17.931Z2025-04-21T16:53:17.931ZS-EPMC95505083622624810.1155/2022/8303409