{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Jagiello A"],"funding":["Edwards Lifesciences","National Institutes of Health Office of the Director","National Institutes of Health","NIH HHS","National Science Foundation"],"pagination":["39-47"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9552552"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["141"],"pubmed_abstract":["Cells are known to constantly interact with their local extracellular matrix (ECM) and respond to a variety of biochemical and mechanical cues received from the ECM. Nonetheless, comprehensive understanding of cell-ECM interactions has been elusive. Many studies rely on analysis of cell behavior on 2D substrates, which do not reflect a natural cell environment. Further, lack of dynamic control over local stiffness anisotropies and fiber alignment hinders progress in studies in naturally derived fibrous 3D cultures. Here, we present a cell-safe method of patterned photocrosslinking, which can aid in studying biological hypotheses related to mechanotransduction in 3D hydrogels. As previously described by our group, ruthenium-catalyzed photocrosslinking (RCP) of selected ECM regions promotes "],"journal":["Acta biomaterialia"],"pubmed_title":["Patterned photocrosslinking to establish stiffness anisotropies in fibrous 3D hydrogels."],"pmcid":["PMC9552552"],"funding_grant_id":["1S10OD025064-01A1","S10 OD025064","DMS-1953410"],"pubmed_authors":["Botvinick E","Castillo U","Jagiello A","Hu Q"],"additional_accession":[]},"is_claimable":false,"name":"Patterned photocrosslinking to establish stiffness anisotropies in fibrous 3D hydrogels.","description":"Cells are known to constantly interact with their local extracellular matrix (ECM) and respond to a variety of biochemical and mechanical cues received from the ECM. Nonetheless, comprehensive understanding of cell-ECM interactions has been elusive. Many studies rely on analysis of cell behavior on 2D substrates, which do not reflect a natural cell environment. Further, lack of dynamic control over local stiffness anisotropies and fiber alignment hinders progress in studies in naturally derived fibrous 3D cultures. Here, we present a cell-safe method of patterned photocrosslinking, which can aid in studying biological hypotheses related to mechanotransduction in 3D hydrogels. As previously described by our group, ruthenium-catalyzed photocrosslinking (RCP) of selected ECM regions promotes ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Mar","modification":"2025-04-29T11:02:35.534Z","creation":"2025-04-06T19:48:00.382Z"},"accession":"S-EPMC9552552","cross_references":{"pubmed":["34971786"],"doi":["10.1016/j.actbio.2021.12.028"]}}