{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Xiu Z"],"funding":["Priority Academic Program Development of Jiangsu Higher Education Institutions","National Natural Science Foundation of China"],"pagination":["5424411"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9553401"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["2022"],"pubmed_abstract":["<h4>Objective</h4>Local radiotherapy may cause distant tumor regression via inducing immunogenic cell death (ICD). Here, we investigated the effect of curcumin on ionizing radiation-induced immunogenic cell death in normoxic or hypoxic glioma cells and its mechanism in vitro and vivo.<h4>Methods</h4>Hypoxic or normoxic glioma cell apoptosis and the cell surface exposure of calreticulin (CRT) were detected by flow cytometry. Extracellular ATP and HSP70 were measured by chemiluminescence assay and ELISA, respectively. Endoplasmic reticulum (ER) stress protein levels were detected by western blot. Moreover, the induction of bona fide ICD was detected by vaccination assays in mice bearing glioma model. Spleen lymphocytes and tumor-infiltrating lymphocyte subsets were analyzed by flow cytometry"],"journal":["Oxidative medicine and cellular longevity"],"pubmed_title":["Curcumin Enhanced Ionizing Radiation-Induced Immunogenic Cell Death in Glioma Cells through Endoplasmic Reticulum Stress Signaling Pathways."],"pmcid":["PMC9553401"],"funding_grant_id":["32171234","81874080","31870844"],"pubmed_authors":["Yang W","Yang Y","He Y","Xiu Z","Sun T","Yang S","Xue X"],"additional_accession":[]},"is_claimable":false,"name":"Curcumin Enhanced Ionizing Radiation-Induced Immunogenic Cell Death in Glioma Cells through Endoplasmic Reticulum Stress Signaling Pathways.","description":"<h4>Objective</h4>Local radiotherapy may cause distant tumor regression via inducing immunogenic cell death (ICD). Here, we investigated the effect of curcumin on ionizing radiation-induced immunogenic cell death in normoxic or hypoxic glioma cells and its mechanism in vitro and vivo.<h4>Methods</h4>Hypoxic or normoxic glioma cell apoptosis and the cell surface exposure of calreticulin (CRT) were detected by flow cytometry. Extracellular ATP and HSP70 were measured by chemiluminescence assay and ELISA, respectively. Endoplasmic reticulum (ER) stress protein levels were detected by western blot. Moreover, the induction of bona fide ICD was detected by vaccination assays in mice bearing glioma model. Spleen lymphocytes and tumor-infiltrating lymphocyte subsets were analyzed by flow cytometry","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-04-12T15:40:01.955Z","creation":"2025-02-19T01:01:59.275Z"},"accession":"S-EPMC9553401","cross_references":{"pubmed":["36238646"],"doi":["10.1155/2022/5424411"]}}