{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Long Y"],"funding":["The Yunnan Applied Basic Research Projects","grants from the NSFC/RGC Joint Research Project","Natural Science Foundation of Guangdong Province","National Natural Science Foundation of China","Shenzhen Key Laboratory of Viral Oncology","the Sanming Project of Medicine in Shenzhen","National Key Research and Development Program of China"],"pagination":["301"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9558416"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["41(1)"],"pubmed_abstract":["Background
Early metastasis is a key factor contributing to poor breast cancer (BC) prognosis. Circulating tumor cells (CTCs) are regarded as the precursor cells of metastasis, which are ultimately responsible for the main cause of death in BC. However, to date molecular mechanisms underlying CTC formation in BC have been insufficiently defined.Methods
RNA-seq was carried out in primary tissues from early-stage BC patients (with CTCs≥5 and CTCs = 0, respectively) and the validation study was conducted in untreated 80 BC patients. Multiple in vitro and in vivo models were used in functional studies. Luciferase reporter, ChIP-seq, CUT&Tag-seq, and GST-pulldown, etc. were utilized in mechanistic studies. CTCs were counted by the CanPatrol™ CTC classification system or LiquidBi"],"journal":["Journal of experimental & clinical cancer research : CR"],"pubmed_title":["FOXD1-dependent RalA-ANXA2-Src complex promotes CTC formation in breast cancer."],"pmcid":["PMC9558416"],"funding_grant_id":["81830087 and U2102203","202101AS070050","N_HKU735/18 and 81861168033","2020A1515010081, 2021A1515010713","ZDSYS201707311140430","2020YFA0112300 and 2018YFC2000400","SZSM201612023"],"pubmed_authors":["Deng S","Guo X","Lyu X","Chen L","He M","Wang F","Zhou W","Chen C","Li X","Qian Z","Long Y","Faleti OD","Luo X","Chong T","Zhao H"],"additional_accession":[]},"is_claimable":false,"name":"FOXD1-dependent RalA-ANXA2-Src complex promotes CTC formation in breast cancer.","description":"Background
Early metastasis is a key factor contributing to poor breast cancer (BC) prognosis. Circulating tumor cells (CTCs) are regarded as the precursor cells of metastasis, which are ultimately responsible for the main cause of death in BC. However, to date molecular mechanisms underlying CTC formation in BC have been insufficiently defined.Methods
RNA-seq was carried out in primary tissues from early-stage BC patients (with CTCs≥5 and CTCs = 0, respectively) and the validation study was conducted in untreated 80 BC patients. Multiple in vitro and in vivo models were used in functional studies. Luciferase reporter, ChIP-seq, CUT&Tag-seq, and GST-pulldown, etc. were utilized in mechanistic studies. CTCs were counted by the CanPatrol™ CTC classification system or LiquidBi","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-19T20:37:23.375Z","creation":"2025-04-19T20:37:23.375Z"},"accession":"S-EPMC9558416","cross_references":{"pubmed":["36229838"],"doi":["10.1186/s13046-022-02504-0"]}}