{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["20(1)"],"submitter":["Duan Y"],"pubmed_abstract":["<h4>Background</h4>Hypertrophy of ligamentum flavum (HLF) is the mainly cause of lumbar spinal stenosis (LSS), but the precise mechanism of HLF formation has not been fully elucidated. Emerging evidence indicates that transcription factor 7 (TCF7) is the key downstream functional molecule of Wnt/β-catenin signaling, which participated in regulating multiple biological processes. However, the role and underlying mechanism of TCF7 in HLF is still unclear.<h4>Methods</h4>We used mRNAs sequencing analysis of human LF and subsequent confirmation with RT-qPCR, western blot and immunohistochemistry to identified the TCF7 in HLF tissues and cells. Then effect of TCF7 on HLF progression was investigated both in vitro and in vivo. Mechanically, chromatin immunoprecipitation, dual-luciferase reporter"],"journal":["Journal of translational medicine"],"pagination":["468"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9558422"],"repository":["biostudies-literature"],"pubmed_title":["TCF7/SNAI2/miR-4306 feedback loop promotes hypertrophy of ligamentum flavum."],"pmcid":["PMC9558422"],"pubmed_authors":["Li J","Qiu S","Cao Y","Ni S","Duan Y"],"additional_accession":[]},"is_claimable":false,"name":"TCF7/SNAI2/miR-4306 feedback loop promotes hypertrophy of ligamentum flavum.","description":"<h4>Background</h4>Hypertrophy of ligamentum flavum (HLF) is the mainly cause of lumbar spinal stenosis (LSS), but the precise mechanism of HLF formation has not been fully elucidated. Emerging evidence indicates that transcription factor 7 (TCF7) is the key downstream functional molecule of Wnt/β-catenin signaling, which participated in regulating multiple biological processes. However, the role and underlying mechanism of TCF7 in HLF is still unclear.<h4>Methods</h4>We used mRNAs sequencing analysis of human LF and subsequent confirmation with RT-qPCR, western blot and immunohistochemistry to identified the TCF7 in HLF tissues and cells. Then effect of TCF7 on HLF progression was investigated both in vitro and in vivo. Mechanically, chromatin immunoprecipitation, dual-luciferase reporter","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-26T22:05:11.976Z","creation":"2025-04-06T17:05:38.852Z"},"accession":"S-EPMC9558422","cross_references":{"pubmed":["36224570"],"doi":["10.1186/s12967-022-03677-0"]}}