{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Sanders JG"],"funding":["NIAID NIH HHS","Foundation for the National Institutes of Health","National Institute of General Medical Sciences","NIGMS NIH HHS"],"pagination":["212"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9558970"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["23(1)"],"pubmed_abstract":["Earth's environments harbor complex consortia of microbes that affect processes ranging from host health to biogeochemical cycles. Understanding their evolution and function is limited by an inability to isolate genomes in a high-throughput manner. Here, we present a workflow for bacterial whole-genome sequencing using open-source labware and the OpenTrons robotics platform, reducing costs to approximately $10 per genome. We assess genomic diversity within 45 gut bacterial species from wild-living chimpanzees and bonobos. We quantify intraspecific genomic diversity and reveal divergence of homologous plasmids between hosts. This enables population genetic analyses of bacterial strains not currently possible with metagenomic data alone."],"journal":["Genome biology"],"pubmed_title":["A low-cost genomics workflow enables isolate screening and strain-level analyses within microbiomes."],"pmcid":["PMC9558970"],"funding_grant_id":["R35 GM138284","T32 AI145821"],"pubmed_authors":["Lonsdorf EV","Morgan DB","Sanz CM","Hahn BH","Sanders JG","Peeters M","Mjungu D","Yan W","Hart JA","Moeller AH"],"additional_accession":[]},"is_claimable":false,"name":"A low-cost genomics workflow enables isolate screening and strain-level analyses within microbiomes.","description":"Earth's environments harbor complex consortia of microbes that affect processes ranging from host health to biogeochemical cycles. Understanding their evolution and function is limited by an inability to isolate genomes in a high-throughput manner. Here, we present a workflow for bacterial whole-genome sequencing using open-source labware and the OpenTrons robotics platform, reducing costs to approximately $10 per genome. We assess genomic diversity within 45 gut bacterial species from wild-living chimpanzees and bonobos. We quantify intraspecific genomic diversity and reveal divergence of homologous plasmids between hosts. This enables population genetic analyses of bacterial strains not currently possible with metagenomic data alone.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-19T20:37:53.631Z","creation":"2025-04-19T20:37:53.631Z"},"accession":"S-EPMC9558970","cross_references":{"pubmed":["36224660"],"doi":["10.1186/s13059-022-02777-w"]}}