biostudies-literatureZhang LNational Natural Science Foundation of China953974https://www.ebi.ac.uk/biostudies/studies/S-EPMC9560764biostudies-literatureUnknown9Mammalian bombesin-like neuropeptides (BLPs) play an important role in regulation of physiological and pathophysiological processes. Frog skin-derived BLPs, of smaller size and diverse lengths and sequences at their N-terminus, have attracted the attention of many researchers. However, these N-terminal variants and the receptors modulating their pharmacological actions are poorly studied and less understood. In this study, two BLPs, namely, [Asn³, Lys⁶, Thr¹⁰, Phe¹³]3-14-bombesin and [Asn³, Lys⁶, Phe¹³]3-14-bombesin with primary structures NLGKQWATGHFM and NLGKQWAVGHFM were isolated from the skin secretion of hybrid <i>Pelophylax</i> kl. <i>esculentus</i>. Both BLPs share a similar primary structure with only a siFrontiers in molecular biosciencesTwo novel bombesin-like neuropeptides from the skin secretion of <i>Pelophylax</i> kl. <i>esculentus</i>: <i>Ex vivo</i> pharmacological characterization on rat smooth muscle types.PMC956076432002344Zhou MXi XMa CLiu MShaw CZhang LChen XChen CZou WChen TWang LfalseTwo novel bombesin-like neuropeptides from the skin secretion of <i>Pelophylax</i> kl. <i>esculentus</i>: <i>Ex vivo</i> pharmacological characterization on rat smooth muscle types.Mammalian bombesin-like neuropeptides (BLPs) play an important role in regulation of physiological and pathophysiological processes. Frog skin-derived BLPs, of smaller size and diverse lengths and sequences at their N-terminus, have attracted the attention of many researchers. However, these N-terminal variants and the receptors modulating their pharmacological actions are poorly studied and less understood. In this study, two BLPs, namely, [Asn³, Lys⁶, Thr¹⁰, Phe¹³]3-14-bombesin and [Asn³, Lys⁶, Phe¹³]3-14-bombesin with primary structures NLGKQWATGHFM and NLGKQWAVGHFM were isolated from the skin secretion of hybrid <i>Pelophylax</i> kl. <i>esculentus</i>. Both BLPs share a similar primary structure with only a si2022-01-01T00:00:00Z20222026-05-28T07:04:23.114Z2025-04-06T09:24:20.496ZS-EPMC95607643625001610.3389/fmolb.2022.953974