{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Watanabe M"],"funding":["NICHD NIH HHS","NIDA NIH HHS","Howard Hughes Medical Institute","NIMH NIH HHS","NINDS NIH HHS"],"pagination":["2220-2238"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9561534"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["17(10)"],"pubmed_abstract":["Telencephalic organoids generated from human pluripotent stem cells (hPSCs) are a promising system for studying the distinct features of the developing human brain and the underlying causes of many neurological disorders. While organoid technology is steadily advancing, many challenges remain, including potential batch-to-batch and cell-line-to-cell-line variability, and structural inconsistency. Here, we demonstrate that a major contributor to cortical organoid quality is the way hPSCs are maintained prior to differentiation. Optimal results were achieved using particular fibroblast-feeder-supported hPSCs rather than feeder-independent cells, differences that were reflected in their transcriptomic states at the outset. Feeder-supported hPSCs displayed activation of diverse transforming growth factor β (TGFβ) superfamily signaling pathways and increased expression of genes connected to naive pluripotency. We further identified combinations of TGFβ-related growth factors that are necessary and together sufficient to impart broad telencephalic organoid competency to feeder-free hPSCs and enhance the formation of well-structured brain tissues suitable for disease modeling."],"journal":["Stem cell reports"],"pubmed_title":["TGFβ superfamily signaling regulates the state of human stem cell pluripotency and capacity to create well-structured telencephalic organoids."],"pmcid":["PMC9561534"],"funding_grant_id":["R01 DA051897","R01 NS089817","P50 HD103557","R01 MH130061","R01 MH121521","R01 MH123922","K99 HD096105","P30 NS062691","U54 HD087101","R01 HD098387","R00 HD096105"],"pubmed_authors":["Buth JE","Baliaouri NV","Pearson CA","Gandal MJ","Torre-Ubieta L","Gu W","Collier AJ","Christofk HR","Elahi LS","Kurdian A","Turcios F","Chen D","Clark AT","Plath K","Dunn N","Haney JR","Vishlaghi N","Watanabe M","Novitch BG","Sabri S","Kornblum HI","Miranda OA"],"additional_accession":[]},"is_claimable":false,"name":"TGFβ superfamily signaling regulates the state of human stem cell pluripotency and capacity to create well-structured telencephalic organoids.","description":"Telencephalic organoids generated from human pluripotent stem cells (hPSCs) are a promising system for studying the distinct features of the developing human brain and the underlying causes of many neurological disorders. While organoid technology is steadily advancing, many challenges remain, including potential batch-to-batch and cell-line-to-cell-line variability, and structural inconsistency. Here, we demonstrate that a major contributor to cortical organoid quality is the way hPSCs are maintained prior to differentiation. Optimal results were achieved using particular fibroblast-feeder-supported hPSCs rather than feeder-independent cells, differences that were reflected in their transcriptomic states at the outset. Feeder-supported hPSCs displayed activation of diverse transforming growth factor β (TGFβ) superfamily signaling pathways and increased expression of genes connected to naive pluripotency. We further identified combinations of TGFβ-related growth factors that are necessary and together sufficient to impart broad telencephalic organoid competency to feeder-free hPSCs and enhance the formation of well-structured brain tissues suitable for disease modeling.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2024-11-21T06:46:30.147Z","creation":"2024-11-21T06:46:30.147Z"},"accession":"S-EPMC9561534","cross_references":{"pubmed":["36179695"],"doi":["10.1016/j.stemcr.2022.08.013"]}}