{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Guan D"],"pubmed_abstract":["To identify and annotate transcript isoforms in the chicken genome, we generated Nanopore long-read sequencing data from 68 samples that encompassed 19 diverse tissues collected from experimental adult male and female White Leghorn chickens. More than 23.8 million reads with mean read length of 790 bases and average quality of 18.2 were generated. The annotation and subsequent filtering resulted in the identification of 55,382 transcripts at 40,547 loci with mean length of 1,700 bases. We predicted 30,967 coding transcripts at 19,461 loci, and 16,495 lncRNA transcripts at 15,512 loci. Compared to existing reference annotations, we found ∼52% of annotated transcripts could be partially or fully matched while ∼47% were novel. Seventy percent of novel transcripts were potentially transcribed "],"journal":["Frontiers in genetics"],"pagination":["997460"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9561881"],"repository":["biostudies-literature"],"pubmed_title":["Prediction of transcript isoforms in 19 chicken tissues by Oxford Nanopore long-read sequencing."],"pmcid":["PMC9561881"],"pubmed_authors":["Halstead MM","Islas-Trejo AD","Goszczynski DE","Guan D","Cheng HH","Zhou H","Ross PJ"],"additional_accession":[]},"is_claimable":false,"name":"Prediction of transcript isoforms in 19 chicken tissues by Oxford Nanopore long-read sequencing.","description":"To identify and annotate transcript isoforms in the chicken genome, we generated Nanopore long-read sequencing data from 68 samples that encompassed 19 diverse tissues collected from experimental adult male and female White Leghorn chickens. More than 23.8 million reads with mean read length of 790 bases and average quality of 18.2 were generated. The annotation and subsequent filtering resulted in the identification of 55,382 transcripts at 40,547 loci with mean length of 1,700 bases. We predicted 30,967 coding transcripts at 19,461 loci, and 16,495 lncRNA transcripts at 15,512 loci. Compared to existing reference annotations, we found ∼52% of annotated transcripts could be partially or fully matched while ∼47% were novel. Seventy percent of novel transcripts were potentially transcribed ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022","modification":"2026-06-01T02:10:08.222Z","creation":"2025-02-19T01:28:19.413Z"},"accession":"S-EPMC9561881","cross_references":{"pubmed":["36246588"],"doi":["10.3389/fgene.2022.997460"]}}