{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Kim YJ"],"funding":["Korea Institute for Advancement of Technology","National Research Foundation of Korea"],"pagination":["29535-29542"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9562052"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["12(45)"],"pubmed_abstract":["Herein, we report a sensitive and selective enzyme-linked aptamer-based sandwich assay (ELASA) to detect <i>Plasmodium falciparum</i> lactate dehydrogenase (<i>Pf</i>LDH), which is an attractive biomarker for malaria diagnosis and antimalarial medication. We performed the sandwich assay with a single aptamer sequence, called 2008s, owing to the structural properties of the <i>Pf</i>LDH tetramer instead of using a conventional sandwich assay with two different aptamers (or antibodies) for capturing and probing a target molecule. First, the biotinylated <i>Pf</i>LDH aptamer was linked with immobilized streptavidin on a microwell plate for binding flexibility, and then <i>Pf</i>LDH was bound to the aptamer. Next, a horseradish peroxidase-conjugated aptamer of the same sequence was used to analyze <i>Pf</i>LDH quantitatively. Using this approach, the limit of detection (LOD) of <i>Pf</i>LDH with the naked eye was 100 ng mL<sup>-1</sup>, and the LOD and limit of quantification from the absorbance measurements were 34.9 ng mL<sup>-1</sup> and 95.5 ng mL<sup>-1</sup>, respectively, based on <i>Pf</i>LDH spiked blood samples. Our proposed method selectively binds <i>Pf</i>LDH, not human lactate dehydrogenase. Therefore, this method may be a valuable tool for diagnosing, monitoring, and quarantining malaria cases easily and rapidly."],"journal":["RSC advances"],"pubmed_title":["Enzyme-linked aptamer-based sandwich assay (ELASA) for detecting <i>Plasmodium falciparum</i> lactate dehydrogenase, a malarial biomarker."],"pmcid":["PMC9562052"],"funding_grant_id":["2021R1F1A1062994","P0017805","2021RIS-001"],"pubmed_authors":["Kim YJ","Choi JW"],"additional_accession":[]},"is_claimable":false,"name":"Enzyme-linked aptamer-based sandwich assay (ELASA) for detecting <i>Plasmodium falciparum</i> lactate dehydrogenase, a malarial biomarker.","description":"Herein, we report a sensitive and selective enzyme-linked aptamer-based sandwich assay (ELASA) to detect <i>Plasmodium falciparum</i> lactate dehydrogenase (<i>Pf</i>LDH), which is an attractive biomarker for malaria diagnosis and antimalarial medication. We performed the sandwich assay with a single aptamer sequence, called 2008s, owing to the structural properties of the <i>Pf</i>LDH tetramer instead of using a conventional sandwich assay with two different aptamers (or antibodies) for capturing and probing a target molecule. First, the biotinylated <i>Pf</i>LDH aptamer was linked with immobilized streptavidin on a microwell plate for binding flexibility, and then <i>Pf</i>LDH was bound to the aptamer. Next, a horseradish peroxidase-conjugated aptamer of the same sequence was used to analyze <i>Pf</i>LDH quantitatively. Using this approach, the limit of detection (LOD) of <i>Pf</i>LDH with the naked eye was 100 ng mL<sup>-1</sup>, and the LOD and limit of quantification from the absorbance measurements were 34.9 ng mL<sup>-1</sup> and 95.5 ng mL<sup>-1</sup>, respectively, based on <i>Pf</i>LDH spiked blood samples. Our proposed method selectively binds <i>Pf</i>LDH, not human lactate dehydrogenase. Therefore, this method may be a valuable tool for diagnosing, monitoring, and quarantining malaria cases easily and rapidly.","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-05T14:54:35.471Z","creation":"2025-04-05T14:54:35.471Z"},"accession":"S-EPMC9562052","cross_references":{"pubmed":["36320752"],"doi":["10.1039/d2ra03796c"]}}