biostudies-literatureRabaglino MBInnovation Fund DenmarkMilk Levy Foundation1113-1124https://www.ebi.ac.uk/biostudies/studies/S-EPMC9562124biostudies-literatureUnknown107(4)In cattle, the in vitro production (IVP) of embryos is becoming more relevant than embryos produced in vivo, i.e. after multiple ovulation and embryo transfer (MOET). However, the effects of IVP on the developmental programming of specific organs in the postnatal calves are yet unknown. Previously, we reported an epigenomic and transcriptomic profile of the hypothalamus-pituitary-testicular axis compatible with its earlier activation in IVP calves compared to MOET animals. Here, we studied the hepatic and muscular epigenome and transcriptome of those same male dairy calves (n = 4 per group). Tissue samples from liver and semitendinosus muscle were obtained at 3 months of age, and the extracted gDNA and RNA were sequenced through whole-genome bisulfite sequencing and RNA-sequencing, respectBiology of reproductionIn vitro- and in vivo-produced male dairy calves show molecular differences in the hepatic and muscular energy regulation†.PMC95621247045-00013BHyttel PKadarmideen HNRabaglino MBSecher JBfalseIn vitro- and in vivo-produced male dairy calves show molecular differences in the hepatic and muscular energy regulation†.In cattle, the in vitro production (IVP) of embryos is becoming more relevant than embryos produced in vivo, i.e. after multiple ovulation and embryo transfer (MOET). However, the effects of IVP on the developmental programming of specific organs in the postnatal calves are yet unknown. Previously, we reported an epigenomic and transcriptomic profile of the hypothalamus-pituitary-testicular axis compatible with its earlier activation in IVP calves compared to MOET animals. Here, we studied the hepatic and muscular epigenome and transcriptome of those same male dairy calves (n = 4 per group). Tissue samples from liver and semitendinosus muscle were obtained at 3 months of age, and the extracted gDNA and RNA were sequenced through whole-genome bisulfite sequencing and RNA-sequencing, respect2022-01-01T00:00:00Z2022 Oct2026-05-27T19:08:14.804Z2024-11-06T07:17:25.468ZS-EPMC95621243576640610.1093/biolre/ioac131