biostudies-literatureTimsina JNIA NIH HHS193-207https://www.ebi.ac.uk/biostudies/studies/S-EPMC9562128biostudies-literatureUnknown89(1)<h4>Background</h4>The SOMAscan assay has an advantage over immunoassay-based methods because it measures a large number of proteins in a cost-effective manner. However, the performance of this technology compared to the routinely used immunoassay techniques needs to be evaluated.<h4>Objective</h4>We performed comparative analyses of SOMAscan and immunoassay-based protein measurements for five cerebrospinal fluid (CSF) proteins associated with Alzheimer's disease (AD) and neurodegeneration: NfL, Neurogranin, sTREM2, VILIP-1, and SNAP-25.<h4>Methods</h4>We compared biomarkers measured in ADNI (N = 689), Knight-ADRC (N = 870), DIAN (N = 115), and Barcelona-1 (N = 92) cohorts. Raw protein values were transformed using z-score in order to combine measures from the different studies. sTREM2 andJournal of Alzheimer's disease : JADComparative Analysis of Alzheimer's Disease Cerebrospinal Fluid Biomarkers Measurement by Multiplex SOMAscan Platform and Immunoassay-Based Approach.PMC9562128R01 AG057777R01 AG044546P30 AG066444U01 AG058922RF1 AG074007U01 AG024904P01 AG026276RF1 AG058501U19 AG032438P01 AG003991RF1 AG053303Fagan AMGomez-Fonseca DBateman RJAguilar MWang LAlzheimer’s Disease Neuroimaging InitiativeSchindler SEDominantly Inherited Alzheimer Network ConsortiaFarlow MMori HPerrin RJSung YJAlvarez IHerries ETimsina JHassenstab JPastor PDo AWestern DMorris JCMoulder KCruchaga CHenson RLXiong CVoglein JChhatwal JfalseComparative Analysis of Alzheimer's Disease Cerebrospinal Fluid Biomarkers Measurement by Multiplex SOMAscan Platform and Immunoassay-Based Approach.<h4>Background</h4>The SOMAscan assay has an advantage over immunoassay-based methods because it measures a large number of proteins in a cost-effective manner. However, the performance of this technology compared to the routinely used immunoassay techniques needs to be evaluated.<h4>Objective</h4>We performed comparative analyses of SOMAscan and immunoassay-based protein measurements for five cerebrospinal fluid (CSF) proteins associated with Alzheimer's disease (AD) and neurodegeneration: NfL, Neurogranin, sTREM2, VILIP-1, and SNAP-25.<h4>Methods</h4>We compared biomarkers measured in ADNI (N = 689), Knight-ADRC (N = 870), DIAN (N = 115), and Barcelona-1 (N = 92) cohorts. Raw protein values were transformed using z-score in order to combine measures from the different studies. sTREM2 and2022-01-01T00:00:00Z20222026-06-04T05:56:18.588Z2026-05-06T03:08:03.394ZS-EPMC95621283587134610.3233/jad-22039910.3233/JAD-220399