biostudies-literatureBrocco DItalian Ministry of University and Research (MIUR), "Progetti di Ricerca di Interesse Nazionale" (PRIN)University "G.d'Annunzio" of Chieti-Pescara, "Search for Excellence 2020" to P.S.&quot;4748https://www.ebi.ac.uk/biostudies/studies/S-EPMC9562679biostudies-literatureUnknown14(19)Pancreatic cancer (PC) is one of the leading causes of cancer-related death worldwide. Identification of novel tumor biomarkers is highly advocated in PC to optimize personalized treatment algorithms. Blood-circulating extracellular vesicles hold promise for liquid biopsy application in cancer. We used an optimized flow cytometry protocol to study leukocyte-derived EVs (CD45+) and PD-L1+ EVs in blood from 56 pancreatic cancer patients and 48 healthy controls (HCs). Our results show that PC patients presented higher blood levels of total EVs (<i>p</i> = 0.0003), leukocyte-derived EVs (LEVs) (<i>p</i> = 0.001) and PD-L1+ EVs (<i>p</i> = 0.01), as compared with HCs. Interestingly, a blood concentration of LEVs at baseline was independently associated with improved overall survival in patientsCancersHigh Blood Concentration of Leukocyte-Derived Extracellular Vesicles Is Predictive of Favorable Clinical Outcomes in Patients with Pancreatic Cancer: Results from a Multicenter Prospective Study.PMC95626792017EKMFTN_005CUP D59C20000680005Zappacosta BDe Tursi MLuisi DMarchisio MDi Sebastiano PDe Bellis DVeschi SBrocco DCama ADi Mola FFDe Fabritiis SDi Marino PAngelone ALanuti PGrassadonia ADi Gregorio PLellis LSimeone PFlorio RGrottola TCaporale MVerginelli FTinari NfalseHigh Blood Concentration of Leukocyte-Derived Extracellular Vesicles Is Predictive of Favorable Clinical Outcomes in Patients with Pancreatic Cancer: Results from a Multicenter Prospective Study.Pancreatic cancer (PC) is one of the leading causes of cancer-related death worldwide. Identification of novel tumor biomarkers is highly advocated in PC to optimize personalized treatment algorithms. Blood-circulating extracellular vesicles hold promise for liquid biopsy application in cancer. We used an optimized flow cytometry protocol to study leukocyte-derived EVs (CD45+) and PD-L1+ EVs in blood from 56 pancreatic cancer patients and 48 healthy controls (HCs). Our results show that PC patients presented higher blood levels of total EVs (<i>p</i> = 0.0003), leukocyte-derived EVs (LEVs) (<i>p</i> = 0.001) and PD-L1+ EVs (<i>p</i> = 0.01), as compared with HCs. Interestingly, a blood concentration of LEVs at baseline was independently associated with improved overall survival in patients2022-01-01T00:00:00Z2022 Sep2025-05-29T22:29:39.86Z2025-05-29T22:29:39.86ZS-EPMC95626793623067110.3390/cancers14194748