{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"omics_type":["Unknown"],"volume":["13"],"submitter":["Wijler LA"],"pubmed_abstract":["<h4>Purpose</h4>Muscle-wasting and treatment-related toxicities negatively impact prognosis of colorectal cancer (CRC) patients. Specific nutritional composition might support skeletal muscle and enhance treatment support. In this <i>in vitro</i> study we assess the effect of nutrients EPA, DHA, L-leucine and vitamin D3, as single nutrients or in combination on chemotherapy-treated C2C12-myotubes, and specific CRC-tumor cells.<h4>Materials and methods</h4>Using C2C12-myotubes, the effects of chemotherapy (oxaliplatin, 5-fluorouracil, oxaliplatin+5-fluorouracil and irinotecan) on protein synthesis, cell-viability, caspase-3/7-activity and LDH-activity were assessed. Addition of EPA, DHA, L-leucine and vitamin D3 and their combination (SNCi) were studied in presence of above chemotherapies. "],"journal":["Oncotarget"],"pagination":["1094-1108"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9564364"],"repository":["biostudies-literature"],"pubmed_title":["<i>In vitro</i> chemotherapy-associated muscle toxicity is attenuated with nutritional support, while treatment efficacy is retained."],"pmcid":["PMC9564364"],"pubmed_authors":["Quirindongo H","van Dijk M","Wijler LA","May AM","Dijk FJ","Furber MJW","Kranenburg O","Dorresteijn B","Raats DAE"],"additional_accession":[]},"is_claimable":false,"name":"<i>In vitro</i> chemotherapy-associated muscle toxicity is attenuated with nutritional support, while treatment efficacy is retained.","description":"<h4>Purpose</h4>Muscle-wasting and treatment-related toxicities negatively impact prognosis of colorectal cancer (CRC) patients. Specific nutritional composition might support skeletal muscle and enhance treatment support. In this <i>in vitro</i> study we assess the effect of nutrients EPA, DHA, L-leucine and vitamin D3, as single nutrients or in combination on chemotherapy-treated C2C12-myotubes, and specific CRC-tumor cells.<h4>Materials and methods</h4>Using C2C12-myotubes, the effects of chemotherapy (oxaliplatin, 5-fluorouracil, oxaliplatin+5-fluorouracil and irinotecan) on protein synthesis, cell-viability, caspase-3/7-activity and LDH-activity were assessed. Addition of EPA, DHA, L-leucine and vitamin D3 and their combination (SNCi) were studied in presence of above chemotherapies. ","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-26T22:03:40.385Z","creation":"2024-10-16T02:45:26.85Z"},"accession":"S-EPMC9564364","cross_references":{"pubmed":["36242541"],"doi":["10.18632/oncotarget.28279"]}}