biostudies-literatureUnknown13Wijler LA<h4>Purpose</h4>Muscle-wasting and treatment-related toxicities negatively impact prognosis of colorectal cancer (CRC) patients. Specific nutritional composition might support skeletal muscle and enhance treatment support. In this <i>in vitro</i> study we assess the effect of nutrients EPA, DHA, L-leucine and vitamin D3, as single nutrients or in combination on chemotherapy-treated C2C12-myotubes, and specific CRC-tumor cells.<h4>Materials and methods</h4>Using C2C12-myotubes, the effects of chemotherapy (oxaliplatin, 5-fluorouracil, oxaliplatin+5-fluorouracil and irinotecan) on protein synthesis, cell-viability, caspase-3/7-activity and LDH-activity were assessed. Addition of EPA, DHA, L-leucine and vitamin D3 and their combination (SNCi) were studied in presence of above chemotherapies. Oncotarget1094-1108https://www.ebi.ac.uk/biostudies/studies/S-EPMC9564364biostudies-literature<i>In vitro</i> chemotherapy-associated muscle toxicity is attenuated with nutritional support, while treatment efficacy is retained.PMC9564364Quirindongo Hvan Dijk MWijler LAMay AMDijk FJFurber MJWKranenburg ODorresteijn BRaats DAEfalse<i>In vitro</i> chemotherapy-associated muscle toxicity is attenuated with nutritional support, while treatment efficacy is retained.<h4>Purpose</h4>Muscle-wasting and treatment-related toxicities negatively impact prognosis of colorectal cancer (CRC) patients. Specific nutritional composition might support skeletal muscle and enhance treatment support. In this <i>in vitro</i> study we assess the effect of nutrients EPA, DHA, L-leucine and vitamin D3, as single nutrients or in combination on chemotherapy-treated C2C12-myotubes, and specific CRC-tumor cells.<h4>Materials and methods</h4>Using C2C12-myotubes, the effects of chemotherapy (oxaliplatin, 5-fluorouracil, oxaliplatin+5-fluorouracil and irinotecan) on protein synthesis, cell-viability, caspase-3/7-activity and LDH-activity were assessed. Addition of EPA, DHA, L-leucine and vitamin D3 and their combination (SNCi) were studied in presence of above chemotherapies. 2022-01-01T00:00:00Z2022 Oct2025-04-26T22:03:40.385Z2024-10-16T02:45:26.85ZS-EPMC95643643624254110.18632/oncotarget.28279