{"database":"biostudies-literature","file_versions":[],"scores":null,"additional":{"submitter":["Wang C"],"funding":["The US National Science Foundation","National Natural Science Foundation of China","The China Postdoctoral Science Foundation"],"pagination":["e2208441119"],"full_dataset_link":["https://www.ebi.ac.uk/biostudies/studies/S-EPMC9564942"],"repository":["biostudies-literature"],"omics_type":["Unknown"],"volume":["119(41)"],"pubmed_abstract":["Meiotic recombination is initiated by the SPORULATION 11 (SPO11)-triggered formation of double-strand breaks (DSBs) that usually occur in open chromatin with active transcriptional features in many eukaryotes. However, gene transcription at DSB sites appears to be detrimental for repair, but the regulatory mechanisms governing transcription at meiotic DSB sites are largely undefined in plants. Here, we demonstrate that the largest DNA polymerase epsilon subunit POL2A interacts with SU(VAR)3 to 9 homologs SUVH2 and SUVH9. N-SIM (structured illumination microscopy) observation shows that the colocalization of SUVH2 with the meiotic DSB marker γ-H2AX is dependent on POL2A. RNA-seq of male meiocytes demonstrates that POL2A and SUVH2 jointly repress the expression of 865 genes, which have sever"],"journal":["Proceedings of the National Academy of Sciences of the United States of America"],"pubmed_title":["DNA polymerase epsilon interacts with SUVH2/9 to repress the expression of genes associated with meiotic DSB hotspot in <i>Arabidopsis</i>."],"pmcid":["PMC9564942"],"funding_grant_id":["IOS-1844264","31870293","32000246","31900445","32170334","2019M661343","31925005"],"pubmed_authors":["Huang J","Zhang J","Wang C","You C","Copenhaver GP","Wang Y","Yu Y"],"additional_accession":[]},"is_claimable":false,"name":"DNA polymerase epsilon interacts with SUVH2/9 to repress the expression of genes associated with meiotic DSB hotspot in <i>Arabidopsis</i>.","description":"Meiotic recombination is initiated by the SPORULATION 11 (SPO11)-triggered formation of double-strand breaks (DSBs) that usually occur in open chromatin with active transcriptional features in many eukaryotes. However, gene transcription at DSB sites appears to be detrimental for repair, but the regulatory mechanisms governing transcription at meiotic DSB sites are largely undefined in plants. Here, we demonstrate that the largest DNA polymerase epsilon subunit POL2A interacts with SU(VAR)3 to 9 homologs SUVH2 and SUVH9. N-SIM (structured illumination microscopy) observation shows that the colocalization of SUVH2 with the meiotic DSB marker γ-H2AX is dependent on POL2A. RNA-seq of male meiocytes demonstrates that POL2A and SUVH2 jointly repress the expression of 865 genes, which have sever","dates":{"release":"2022-01-01T00:00:00Z","publication":"2022 Oct","modification":"2025-04-19T00:00:45.558Z","creation":"2025-04-07T11:15:48.613Z"},"accession":"S-EPMC9564942","cross_references":{"pubmed":["36191225"],"doi":["10.1073/pnas.2208441119"]}}